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Expression data from human oligodendroglia cells expressing shRNA targeting ADAR2 or control.

ABSTRACT: Expression data from human oligodendroglia cells expressing shRNA targeting ADAR2 or control.

PROVIDER: PRJNA577824 | ENA |

REPOSITORIES: ENA

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Expression data from human oligodendroglia cells expressing shRNA targeting ADAR2 or control.

Project description:To assess whether antiviral genes are upregulated by ADAR2 knockdown, we performed a comprehensive analysis using a microarray system for oligodendroglia cells introduced shRNA against ADAR2 or control. The inflammation- and immune response-related genes were upregulated in ADAR2-knockdown cells.

2019-10-17 | GSE138927 | GEO

Human Gastric Cancer Cell line MKN45: Non-Target shRNA vs. SET/I2PP2A targeting shRNA

Project description:Transcriptional profiling of MKN45 cells comparing control stably expressing Non-Targeting shRNA cells with stably expressing SET/I2PP2A targeting shRNA cells. Goal was to determine the effects of SET knockdown on MKN45 gene expression.

2016-09-06 | GSE86437 | GEO

Knockdown of RNA deaminase ADAR2 in Th17 cells

Project description:ADAR2 is induced upon T cell activation and its immunological function is not known. Here, we performed RNA-seq on Cas9+ control and Th17 cells expressing the PSIN empty vector control or guide RNAs against the ATG start codon or ADAR2 to identify ADAR2 dependent A-to-I edited transcripts.

2024-12-31 | GSE227152 | GEO

Expression data from human adrenocortical H295R cells expressing a doxycyclin-inducible shRNA targeting CTNNB1

Project description:We used the H295R cell line, human adrenocortical cells, harboring a heterozygous CTNNB1 (beta-catenin) gene mutation affecting the GSK3 beta-phosphorylation site (S45P) and leading to constitutive transcriptional activity of beta-catenin-LEF/TCF. Whole-transcript gene expression was analyzed in three stable clones of H295R cells expressing a doxycycline-inducible shRNA targeting CTNNB1 mRNA and in a control clone, without or with doxycycline for 5 days.

2015-08-03 | E-MTAB-3330 | biostudies-arrayexpress

Genomic expression analysis of K562 cells expressing shRNA targeting lncRNA-IIRX and control cells

Project description:Genomic expression analysis of K562 cells expressing shRNA targeting lncRNA-IIRX and control cells

| PRJNA492752 | ENA

Next Generation Sequencing Analysis of Wild Type, ADAR2 over expressing and ADAR2+SRSF9 overexpresssing HEK 293T cells Transcriptomes

Project description:Purpose: A-to-I RNA editing is critical for many cellular processes. The sites of A-I RNA editing can be identified through RNA-seq and matching the reads to the annotated database like RADAR. The goals of this study is to compare A-I RNA editing profile among wild type, ADAR2 overexpressing and ADAR2+SRSF9 overexpressing 293T cells to evaluate the influence of SRSF9 repressive action on A-I RNA editing Methods: A-I RNA editing profile and gene expression profiles were generated by deep sequencing from all the samples, using NEBNext® Ultra™ Directional RNA Library Prep Kit. The sequence reads that passed quality filters were analyzed for identifying the A-I RNA editing sites using RADAR Database Results: Upon overexpression of ADAR2, we found that 18,174 sites were differentially edited compared to control cells, of which 97.0% showed a significant increase in their editing levels as expected (P < 0.05, Fisher’s test). Furthermore, when we overexpressed both ADAR2 and SRSF9 together, we found that 6,994 sites were differentially edited compared to overexpression of the deaminase alone (P < 0.05, Fisher’s test). Importantly, the editing of 92.5% of these sites was down-regulated, consistent with the function of SRSF9 as a repressor of editing. Conclusions: Our study reports a detailed analysis of the effect of SRSF9 on A-I RNA editing of ADAR2-specific targets. We Conclude that this SRSF9 regulon is significantly enriched for brain-specific editing sites despite our unbiased approach.

2018-06-21 | GSE108126 | GEO

Next Generation Sequencing Analysis of Wild Type, ADAR2 over expressing and ADAR2+SRSF9 overexpresssing SH-Sy5y cells Transcriptomes

2018-06-21 | GSE114970 | GEO

MiRNAinU118_Cells_Inactive_vs_ active_ Adar2

Project description:MicroRNA expression profile of NB U118 cells transfected with a plasmid directing an inactive Adar2 vs an active Adar2 - 3 biological replicates 1 Condition experiment, 3 biological replicates, 2 replicates/array

2015-02-28 | E-GEOD-63694 | biostudies-arrayexpress

Genomic expression analysis of K562 cells expressing shRNA targeting lncRNA-IIRX and control cells

Project description:LncRNA-IIRX plays critical role in Bcr-Abl-induced tumorigenesis. To discover its mechanisms underlying cellular transformation by Bcr-Abl oncogene, genome-wide mRNA expression was measured by RNA-seq in K562 cells expressing shRNA targeting lncRNA-IIRX and control cells. We identified many genes with differential expression in K562 cells after knocking down lncRNA-IIRX.

2019-04-17 | GSE120337 | GEO

Next Generation Sequencing Analysis of Wild Type, ADAR2 over expressing and ADAR2+SRSF9 overexpresssing SH-Sy5y cells Transcriptomes

Project description:Next Generation Sequencing Analysis of Wild Type, ADAR2 over expressing and ADAR2+SRSF9 overexpresssing SH-Sy5y cells Transcriptomes

| PRJNA473370 | ENA

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