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Small RNA-seq comparing transcriptome (small RNAs) of spermatozoa from mice fed either a control-Diet or a Western-diet for One or Five successive generations

ABSTRACT: Small RNA-seq comparing transcriptome (small RNAs) of spermatozoa from mice fed either a control-Diet or a Western-diet for One or Five successive generations

PROVIDER: PRJNA577941 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10294583.fastq.gz	Fastqsanger.gz
	SRR10294584.fastq.gz	Fastqsanger.gz
	SRR10294585.fastq.gz	Fastqsanger.gz
	SRR10294586.fastq.gz	Fastqsanger.gz
	SRR10294587.fastq.gz	Fastqsanger.gz

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Small RNA-seq comparing transcriptome (small RNAs) of spermatozoa from mice fed either a control-Diet or a Western-diet for One or Five successive generations

Project description:Gene expression analysis of sperm small RNA obtained from mice fed either a control or Western diets for one or five successive generations through the paternal lineage. The aim of the study is to identify differentially expressed small RNA in sperm of obese males which might be involved in the long-term epigenetic inheritance of obesity induced by the maintenance of High Fat Diet feeding for 5 successive generations.

2021-03-31 | GSE138989 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of epididymal adipose tissue transcriptomes from mice fed either a control-diet or aWestern-diet for one or five successive generations

Project description:Purpose: Successive paternal generations of unhealthy diet induce a fat mass increase. The goal of this study is to compare epididymal adipose tissue transcriptome profiling (RNA-seq) of mice fed either a control-Diet or a High-Fat-Diet for One or Five successive generations. The aim of the study is to identify differentially expressed RNA in epididymal adipose tissue of obese males which might be involved in the exacerbation of induced by the maintenance of High Fat Diet feeding for 5 successive generations. Methods: Epididymal adipose tissue mRNA profiles of 18-months-old mice fed either a control-Diet or a Western-Diet for One or Five successive generations were generated by deep sequencing, in triplicate, using Illumina GAIIx. The sequence reads that passed quality filters were quantitavely analyzed. Reads abundance was evaluated for each gene followed by annotation versus mouse GTF by using the featureCounts function.The R package Edger was used in order to normalize the reads and to identify differentially expressed (DE) genes. Results: RNA-seq data revealed a specific enrichment in gene related to metabolic syndrome traits in male mice obtained after successive paternal generations of Western-Diet.

2021-04-15 | GSE148972 | GEO

Epigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice [BS-seq]

Project description:Transmission of epigenetic information between generations occurs in nematodes, flies and plants, mediated by specialised small RNA pathways, modified histones and DNA methylation. In mammals, evidence supports similar trans- and inter-generational effects although the underlying mechanisms are incompletely understood. Here a luciferase knock-in reporter mouse for the imprinted Dlk1 locus was generated to visualise and track epigenetic fidelity across generations. Exposure to high-fat diet (HFD) in pregnancy provoked sustained re-expression of the normally silent maternal Dlk1 allele in offspring (a loss of imprinting) and increased DNA methylation at the sDMR. In the next generation heterogeneous Dlk1 mis-expression was seen exclusively among animals born to F1-exposed females. Oocytes from these females showed altered gene and miR expression without changes in DNA methylation, and correct imprinting was restored in subsequent generations. Our results illustrate how diet impacts the foetal epigenome, disturbing canonical and non-canonical imprinting mechanisms to modulate the properties of two successive generations of offspring.

2022-04-01 | GSE175537 | GEO

Epigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice [RNA-seq]

2022-04-01 | GSE175536 | GEO

Characterization of the small RNA population in Human spermatozoa

Project description:Small noncoding RNAs (sncRNA) are becoming recognized for their participation in a diverse range of cellular functions. In this study, their global characterization in human spermatozoa from donors with proven fertility was undertaken. Reads were analyzed in two classes, those mapping to unique locations and those that could be aligned to up to 10 genomic locations. All libraries showed comparable distribution of reads between intergenic, intronic and exonic genomic regions. Analysis of the sequences revealed the presence of multiple small RNA classes. The miRNAs retained in spermatozoa were found to be associated with promoter regions, suggestive of a role at the transcriptional level. Piwi-interacting sncRNAs as well as repeat-associated small RNAs were identified for the first time in mature spermatozoa from a mammalian species. Human spermatozoa retain a multifaceted population of small non-coding RNAs. Their presence is consistent with the view that they may function to stabilize the genome as part of the confrontation and consolidation of the genomes at fertilization. Examination of sperm samples from 3 individuals, sequenced individually

2011-10-15 | E-GEOD-21191 | biostudies-arrayexpress

Characterization of the small RNA population in Human spermatozoa

2011-10-16 | GSE21191 | GEO

Small RNA-seq comparing transcriptome (small RNAs) of spermatozoa from rat fed either a standard-Diet (SD) or a High-Protein-Diet (HPD)

Project description:Small RNA-seq comparing transcriptome (small RNAs) of spermatozoa from rat fed either a standard-Diet (SD) or a High-Protein-Diet (HPD)

| PRJNA715449 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of epididymal adipose tissue transcriptomes from mice fed either a control-diet or aWestern-diet for one or five successive generations

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of epididymal adipose tissue transcriptomes from mice fed either a control-diet or aWestern-diet for one or five successive generations

| PRJNA626656 | ENA

Small RNA-seq comparing transcriptome (small RNAs) of spermatozoa from rat fed either a standard-Diet (SD) or a High-Protein-Diet (HPD)

Project description:Gene expression analysis of sperm small RNA obtained from rat fed either a standard (SD) or High Protein Diets (HPD). The aim of the study is to identify differentially expressed small RNA in sperm of SD-fed and HPD-fed males which might be involved in the epigenetic inheritance of glucose sensitivity induced by a High Protein Diet.

2021-05-21 | GSE169157 | GEO

A human-like fat diet is sufficient to induce transgenerational obesity

Project description:The prevalence of obesity has steadily increased since decades. In the meantime, in line with the exposition of populations of from industrialized countries have been exposed to fat-rich diet with high exaggerated ù6/ù3 ratio of ù6- over ù3-polyunsaturated fatty acids. To assess the contribution of dietary fatty acids, male and female mice fed a human-like fat diet were mated randomly and maintained after breeding on the same diet for successive generations. Offspring showed over 4 generations a gradual enhancement in fat mass due to combined hyperplasia and hypertrophy with no change in energy intake. Transgenerational alterations in adipokine levels were accompanied by hyperinsulinemia. When switched to a standard diet, the obese phenotype (termed revHF4) reversed partially. Offspring of revHF4 mice exhibited higher birth weight and faster gain weight compared to control mice. Gene expression analyses of the stromal vascular fraction of adipose tissue over generations revealed i) underexpression of genes favoring angiogenesis and controlling oxidative stress and ii) overexpression of genes involved in inflammation, and in adipogenesis .and in insulin resistance. Thus, under conditions of genome stability, our results show that metabolic imprinting induced by a human-like fat diet is accompanied by a gradual fat mass enhancement, in accordance with the increasing prevalence of obesity observed in humans over generations.

2010-06-01 | GSE16636 | GEO

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