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Single-cell RNA-seq of sorted E11.5 AGM HSCs

ABSTRACT: Single-cell RNA-seq of sorted E11.5 AGM HSCs

PROVIDER: PRJNA578153 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10302700.fastq.gz	Fastqsanger.gz
	SRR10302800.fastq.gz	Fastqsanger.gz
	SRR10302900.fastq.gz	Fastqsanger.gz
	SRR10303000.fastq.gz	Fastqsanger.gz
	SRR10302701.fastq.gz	Fastqsanger.gz

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Single-cell RNA-seq of sorted E11.5 AGM HSCs

Project description:The aim of this study was to analyse the heterogeneity within the first haematopoietic stem cells generated during mouse development using single-cell RNA-Seq. Freshly dissected aorta-gonads-mesohenphros (AGM) regions from E11.5 mouse embryos were dissociated by collagenase and the cells stained for the cell surface markers EPCR and CD45. EPCR+CD45+ cells were index-sorted as single cells into 96-well plates and processed based on the SmartSeq2 protocol. PCA and tSNE analyses identified 2 main subpopulations which are segregated based on differences in cell cycle. Known HSC markers such as Gata2 and Runx1 show little correlation.

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