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De novo methylaton of the unmethylated K. phaffii genome by human DNA methyltransferases (DNMTs)

ABSTRACT: De novo methylaton of the unmethylated K. phaffii genome by human DNA methyltransferases (DNMTs)

PROVIDER: PRJNA578169 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10303600_1.fastq.gz	Fastqsanger.gz
	SRR10303600_2.fastq.gz	Fastqsanger.gz
	SRR10303601_1.fastq.gz	Fastqsanger.gz
	SRR10303601_2.fastq.gz	Fastqsanger.gz
	SRR10303602_1.fastq.gz	Fastqsanger.gz

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De novo methylaton of the unmethylated K. phaffii genome by human DNA methyltransferases (DNMTs) [RNA-seq]

Project description:De novo methylaton of the unmethylated K. phaffii genome by human DNA methyltransferases (DNMTs) [RNA-seq]

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De novo methylaton of the unmethylated K. phaffii genome by human DNA methyltransferases (DNMTs) [BiSulfite-seq]

Project description:De novo methylaton of the unmethylated K. phaffii genome by human DNA methyltransferases (DNMTs) [BiSulfite-seq]

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De novo methylaton of the unmethylated K. phaffii genome by human DNA methyltransferases (DNMTs) [BiSulfite-seq]

Project description:We engineered combinatorial knock-in of human DNMTs in Komagataella phaffii, a yeast species lacking endogenous DNA methylation, to characterize methylation preferences and functional consequences of individual de-novo DNMTs as well as their combinations. Methylation preferences were determined through whole-genome bisulfite sequencing (WGBS) experiments. Effects of knock-in on gene expression were determined by RNA-seq. Measurements of genome-wide methylation produced by distinct de-novo DNMTs permitted determination of methylation preferences and aversions specific to individual DNMTs. Combining methylation and gene expression data provided insight about cellular response to the stress of DNA methylation and about the association between gene methylation patterns and changes in transcriptional output.

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De novo methylaton of the unmethylated K. phaffii genome by human DNA methyltransferases (DNMTs) [RNA-seq]

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De novo methylaton of the unmethylated K. phaffii genome by human DNA methyltransferases (DNMTs)

Project description:This SuperSeries is composed of the SubSeries listed below.

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DNA methyltransferases are complementary in maintaining DNA methylation in embryonic stem cells

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Linking DNA Methyltransferases (DNMTs) to Epigenetic Marks and Nucleosome Structure Genome-Wide in Human Tumor Cells

Project description:Linking DNA Methyltransferases (DNMTs) to Epigenetic Marks and Nucleosome Structure Genome-Wide in Human Tumor Cells

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