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Vitis vinifera

ABSTRACT: Vitis vinifera Genome sequencing

PROVIDER: PRJNA578186 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10331623_1.fastq.gz	Fastqsanger.gz
	SRR10331623_2.fastq.gz	Fastqsanger.gz
	SRR10331624_1.fastq.gz	Fastqsanger.gz
	SRR10331624_2.fastq.gz	Fastqsanger.gz
	SRR10331625_1.fastq.gz	Fastqsanger.gz

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High-throughput sequencing of small RNAs in Vitis vinifera

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Somatic embryogensis of Vitis vinifera exhibits a transcriptomic hourglass pattern

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High-throughput sequencing of small RNAs in Vitis vinifera

Project description:Vitis vinifera endogenous small RNAs Size fractionated small RNA from total RNA extracts of Vitis vinifera leaves, inflorescences, tendrils and small berries were ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

2010-04-29 | E-GEOD-18405 | biostudies-arrayexpress

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Vitis vinifera Transcriptome or Gene expression

Project description:Seedless varieties are of particular importance to the table-grape and raisin industries. Gibberellin (GA) application is widely used in the early stages of seedless berry development to increase berry size and economic value. However, the underlying mechanism of GA induction of berry enlargement is not well understood. Here, RNA-sequencing analysis of âCentennial Seedlessâ (Vitis vinifera L.) berries treated with GA3 12 days after flowering is reported.

2014-12-05 | E-MTAB-4247 | biostudies-arrayexpress

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Project description:Size fractionated small RNA from total RNA extracts of Vitis vinifera leaves were ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Illumina high throughput pyrosequencing. The kit used is TrueSeq Small RNA kit Please see www.illumina.com for details of the sequencing technology.

2016-02-02 | GSE63244 | GEO

microRNA-target RNA pairs revealed by Parallel analysis of RNA ends in Vitis vinifera

Project description:Vitis vinifera RNA degradome Isolated polyadenylated RNA from total RNA extracts of Vitis vinifera leaves, were ligated to 5'-adapter that include san MmeI recognition site. The ligated products were purified again, reverse transcribed and cleaved with MmeI. The 5' fragments were purified from gel and to a 3'- dsDNA adapter and PCR amplified. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

2010-04-29 | E-GEOD-18406 | biostudies-arrayexpress

Transcriptional profiling of Vitis vinifera during grapevine-downy mildew interaction using cDNA microarrays

Project description:Study of gene expression during Plasmopara viticola infection in the resistant Vitis vinifera cultivar 'Regent'. The oomycete fungus Plasmopara viticola (Berk. et Curt.) Berl. et de Toni is responsible for grapevine downy mildew disease. Most of the cultivated grapevines are sensitive to this pathogen, thus requiring intensive fungicide treatments. The molecular basis of resistance to this pathogen is poorly understood. We have carried out a cDNA microarray transcriptome analysis to identify grapevine genes associated with resistance traits. Early transcriptional changes associated with downy mildew infection in the resistant Vitis vinifera cultivar ‘Regent’, when compared to the susceptible cultivar ‘Trincadeira’, were analyzed. Transcript levels were measured at three time-points: 0, 6 and 12 hours post inoculation (hpi). Our data indicate that resistance in V. vinifera ‘Regent’ is induced after infection. This study provides the identification of several candidate genes that may be related to ‘Regent’ defense mechanisms, allowing a better understanding of this cultivar's resistance traits.

2012-04-06 | GSE28439 | GEO

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