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Tumor-derived Schwann-like cells promote neuroblastoma survival through secreted trophic factors

ABSTRACT: Tumor-derived Schwann-like cells promote neuroblastoma survival through secreted trophic factors

PROVIDER: PRJNA578860 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10322367.fastq.gz	Fastqsanger.gz
	SRR10322368.fastq.gz	Fastqsanger.gz
	SRR10322369.fastq.gz	Fastqsanger.gz
	SRR10322370.fastq.gz	Fastqsanger.gz

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Tumor-derived Schwann-like cells promote neuroblastoma survival through secreted trophic factors

Project description:Neuroblastoma (NB) is comprised of a mixture of neuroblastic N-type cancer cells, and Schwannian stromal S-type cells. Sublines of N- and S-type cells were isolated from an early passage of SK-N-SH NB cell line. RNA-Seq analysis was conducted on slected N- and S-type cells in order to define their unique expression profiles.

2021-08-11 | GSE139235 | GEO

Tumour-derived substrate-adherent cells promote neuroblastoma survival through secreted trophic factors.

Project description:Neuroblastoma (NB) is the most common extracranial solid tumour in children. NB is highly heterogeneous and is comprised of a mixture of neuroblastic cancer cells and stromal cells. We previously reported that N-type cells (neuroblastic cells) and S-type cells (substrate-adherent cells) in the SK-N-SH cell line shared almost identical genetic backgrounds. Sublines of N- and S-type cells were isolated from an early passage (P35) of SK-N-SH. Sequencing analysis revealed that all sublines harboured the anaplastic lymphoma kinase (ALK) F1174L mutation, indicating that they were tumour derived. Surprisingly, over 74% resembled S-type cells. In coculture experiments, S-type cells protected N-type cells from apoptosis induced by the oncogenic ALK inhibitor TAE684. Western blotting analyses showed that ALK, protein kinase A (AKT) and STAT3 signalling were stimulated in the cocultures. Furthermore, the conditioned medium from S-type cells activated these downstream signalling molecules in the N-type cells. The activation of STAT3 in the N-type cells was ALK-independent, while AKT was regulated by the ALK activation status. To identify the responsible soluble factors, we used a combination of transcriptomic and proteomic analysis and found that plasminogen activator inhibitor 1, secreted protein acidic and cysteine rich, periostin and galectin-1 were potential mediators of STAT3 signalling. The addition of recombinant proteins to the tumour cells treated with the ALK inhibitor partially enhanced cell viability. Overall, the tumour-derived S-type cells prevented apoptosis in the N-type cells via ALK-independent STAT3 activation triggered by secreted factors. The inhibition of these factors in combination with ALK inhibition could provide a new direction for targeted therapies to treat high-risk NB.

| S-EPMC8334291 | biostudies-literature

Schwann cell condition medium MS

Project description:The effects of Schwann cells on the neuro-stroma niche in pancreatic ductal adenocarcinoma (PDAC) remain to be explored. Here, single-cell RNA-sequencing and spatial transcriptome analysis of PDAC tissues reveals that Schwann cells induce malignant subtypes of tumour cells and cancer-associated fibroblasts. Mass Spectrometry (MS) were performed to detected the potential functional factors secreted by Schwann cells.

2023-06-27 | PXD042556 | Pride

Schwann cell-secreted PGE2 promotes sensory neuron excitability during development [scRNAseq_Dataset1]

Project description:Schwann cell-secreted PGE2 promotes sensory neuron excitability during development [scRNAseq_Dataset1]

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Schwann cell-secreted PGE2 promotes sensory neuron excitability during development [scRNAseq_Dataset2]

Project description:Schwann cell-secreted PGE2 promotes sensory neuron excitability during development [scRNAseq_Dataset2]

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Pro-osteogenic trophic effects by PKA activation in human mesenchymal stromal cells

Project description:Human mesenchymal stromal cells (hMSCs) are able to differentiate into a wide variety of cell types, which makes them an interesting source for tissue engineering applications. On the other hand, these cells also secrete a broad panel of growth factors and cytokines that can exert trophic effects on surrounding tissues. In bone tissue engineering applications, the general assumption is that direct differentiation of hMSCs into osteoblasts accounts for newly observed bone formation in vivo. However, the secretion of bone-specific growth factors, but also pro-angiogenic factors, could also contribute to this process. We recently demonstrated that secretion of bone specific growth factors can be enhanced by treatment of hMSCs with the small molecule db-cAMP (cAMP) and here we investigate the biological activity of these secreted factors. We demonstrate that conditioned medium contains a variety of secreted growth factors, with differences between medium from basic-treated and cAMP-treated hMSCs. We show that conditioned medium from cAMP-treated hMSCs increases proliferation of various cell types and also induces osteogenic differentiation, whereas it has differential effects on migration. Microarray analysis on hMSCs exposed to conditioned medium confirmed upregulation of pathways involved in proliferation as well as osteogenic differentiation. Our data suggests that trophic factors secreted by hMSCs can be tuned for specific applications and that a good balance between differentiation on the one hand and secretion of bone trophic factors on the other, could potentially enhance bone formation for bone tissue engineering applications. For more information check: https://cbit.maastrichtuniversity.nl/

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Malignant Schwann cell precursors mediate intratumoral plasticity in human neuroblastoma

Project description:Malignant Schwann cell precursors mediate intratumoral plasticity in human neuroblastoma

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Single cell RNA seq of the developing murine adrenal gland reveals correlation of Schwann cell precursor signature to neuroblastoma phenotype.

Project description:Neuroblastoma is the most common extracranial solid tumor and accounts for ~10% of pediatric cancer-related deaths. The exact cell-of-origin has yet to be elucidated, but it is generally accepted that neuroblastoma derives from the neural crest and should thus be considered an embryonal malignancy. About 50% of primary neuroblastoma tumors arise in the adrenal gland. Here we present an atlas of the developing mouse adrenal gland at a single cell level. Five main cell cluster groups (medulla, cortex, endothelial, stroma and immune) make up the mouse adrenal gland during fetal development. The medulla group, which is of neural crest origin, is further divided into seven clusters. Of interest is the Schwann cell precursor (SCP) and the neuroblast cluster, a highly cycling cluster that shares markers with sympathoblasts. The signature of the medullary SCP cluster differentiates neuroblastoma patients based on disease phenotype: the SCP signature score anti- correlates with ALK and MYCN expression, two indicators of poor prognosis. Furthermore, a high SCP signature score is associated with better overall survival rates. This study provides an insight in the developing adrenal gland and introduces the SCP gene signature as being of interest for further research in understanding neuroblastoma phenotype.

2021-02-01 | GSE162238 | GEO

Schwann cell-secreted PGE2 promotes sensory neuron excitability during development [RNA-Seq]

Project description:Schwann cell-secreted PGE2 promotes sensory neuron excitability during development [RNA-Seq]

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Gene expression data from human primary cultured Schwann cells

Project description:Schwann cells undergo reprogramming after nerve injury, switching to immature repair phenotype. The goal was to test what genetic perturbations are triggered in Schwann cells with the stimulus from melanoma cell secreted factors. We used microarrays to detail the changes in gene expression of Schwann cells treated with human melanoma conditioned medium.

2019-02-05 | GSE126040 | GEO

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