Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

High-Throughput Translational Profiling with riboPLATE-seq

ABSTRACT: High-Throughput Translational Profiling with riboPLATE-seq

PROVIDER: PRJNA578863 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17007100.fastq.gz	Fastqsanger.gz
	SRR17007101.fastq.gz	Fastqsanger.gz
	SRR17007102.fastq.gz	Fastqsanger.gz
	SRR17007103.fastq.gz	Fastqsanger.gz
	SRR17007104.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 31

Similar Datasets

High-Throughput Translational Profiling with riboPLATE-seq

Project description:Protein synthesis is dysregulated in many diseases, but we lack a systems-level picture of how signaling molecules and RNA binding proteins interact with the translational machinery, largely due to technological limitations. Here we present riboPLATE-seq, a scalable method for generating paired libraries of ribosome-associated and total mRNA. As an extension of the PLATE-seq protocol, riboPLATE-seq utilizes barcoded primers for pooled library preparation, but additionally leverages rRNA immunoprecipitation on whole polysomes to measure ribosome association (RA). We demonstrate the performance of riboPLATE-seq and its utility in detecting translational alterations induced by inhibition of protein kinases.

2019-10-23 | GSE139238 | GEO

Expression profiling by high throughput sequencing

Project description:Expression profiling by high throughput sequencing

| PRJEB7300 | ENA

CoBATCH for high-throughput single-cell epigenomic profiling

Project description:CoBATCH for high-throughput single-cell epigenomic profiling

| PRJNA530991 | ENA

Expression profiling by high throughput sequencing of NDP52

Project description:Expression profiling by high throughput sequencing of NDP52

| PRJNA779423 | ENA

QsRNA-seq: a method for high-throughput profiling and quantifying small RNAs

Project description:QsRNA-seq: a method for high-throughput profiling and quantifying small RNAs

| PRJNA379828 | ENA

mDRIP-seq is a high-throughput method for quantitative R-loop landscape profiling

Project description:Current methods for R-loop profiling need to perform experiments for each sample individually, with consequent limitations in throughput. Here, based on the barcoding strategy, we develop mDRIP-seq, a high-throughput method showing equivalent performance as conventional methods, but with merits of 7-fold less cost and 6-fold less hand-on time per sample. We also show the simplicity and effectiveness of mDRIP-seq for relative and absolute quantitation of genomic R-loop fractions for multiple samples. Together, mDRIP-seq is a high-throughput and cost-efficient method for R-loop mapping and quantitative assessment that can be widely applied to large-scale dynamic profiles of these important structures for diverse organisms.

2024-06-11 | GSE219071 | GEO

mDRIP-seq is a high-throughput method for quantitative R-loop landscape profiling

Project description:mDRIP-seq is a high-throughput method for quantitative R-loop landscape profiling

| PRJNA906979 | ENA

High throughput single cell epigenomic profiling by targeted insertion of promoters (TIP-seq)

Project description:High throughput single cell epigenomic profiling by targeted insertion of promoters (TIP-seq)

| PRJNA779107 | ENA

High throughput gene expression profiling of yeast colonies with microgel-culture Drop-seq

Project description:High throughput gene expression profiling of yeast colonies with microgel-culture Drop-seq

| PRJNA496508 | ENA

IMPC high throughput RNA seq of 1135 lines

Project description:IMPC high throughput RNA seq of 1135 lines