Project description:Immortalized human breast cancer cell lines, MDA-MB-231(ATCC® HTB-26™) and MDA-MB-468(ATCC® HTB-132™), were analyzed via RT-qPCR for transcript expression of several classes of protein factors including cell adhesion, ECM components, cell cycle, cell growth and proliferation, apoptosis, transcription factors and regulators and other genes related to tumor metastasis.
Project description:We performed a transcription factor (TF) ChIP-seq in human embryonal palatal mesenchyme (HEPM; HEPM ATCC ® CRL-1486™) cells with a polyclonal anitbody against TFAP2A ((ab52222, abcam®, Great Britain) in two replicates.
2022-02-02 | GSE169342 | GEO
Project description:Genome sequencing of Bartonella bacilliformis strain KC583
Project description:The mouse melanoma cell line B16-F10 provided by American Type Culture Collection (ATCC® CRL-6475™) were treated with DMSO, G007-LK, WNT or G007-LK+WNT, done in triplicates for a total of 12 samples.
Project description:We previously identified the deubiquitylase USP9X as a G2/M-specific CDC14B interactor (PXD012732). The functional connection of these proteins has been further investigated by a quantitative comparison of USP9X phosphorylation in WT vs CDC14B-overexpressing HEK 293Tcells (ATCC® CRL-3216™). The approach identified USP9X serine 2563 as a specific CDC14B target. Phosphorylation at USP9XS2563 has been confirmed by in-house phosphospecific antibodies.
Project description:MicroRNAs are useful biomarkers for various disease states, and their preservation in formalin-fixed, paraffin-embedded (FFPE) tissue makes them particularly useful for clinicogenetic studies. Although global microRNA expression in FFPE samples is routinely measured with microarrays, the utility of RNA sequencing for such profiling has yet to be established. In this study, to appraise the suitability of RNA sequencing, microRNAs in RNA from pathologic stage I lung adenocarcinoma FFPE samples were quantified with 8x60K Agilent® SurePrint™ G3 Human miRNA 8x60k (release 16.0) microarray and Illumina® HiSeq™ 2000 sequencing platforms.
Project description:Investigation of global gene expression changes in Saccharomyces cerevisiae strain NRRL Y-12632 (ATCC® 18824) grown in media made with asbestos mine tailings-laden water compared to the control grown in media made with double distilled water
