Project description:Here we have used using a pooled proliferation dropout screen with KRAB-dCas9 to determine the contribution of cis-regulatory elements around EGFR to cell fitness in EGFR unamplified (GSC23) and amplified (GBM3565) glioblastoma .
Project description:Prostate cancer (PCa) associated risk SNPs are enriched in cis-regulatory elements (CREs). In this study, we screened 260 CREs that contain at least one PCa risk SNP for essentiality in V16A, 22Rv1 and A549 cells. We tiled the CREs and 14 control regions using 5,873 sgRNAs.
Project description:To delineate cellular pathways underlying cell growth in the absence of sphingolipid syntehsis, we performed a genome-wide CRISPRi screen to identify genetic modifiers of myriocin sensitivity in human K562 cells.