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Genome-wide analysis of histone marks in N1E115 cells

ABSTRACT: Genome-wide analysis of histone marks in N1E115 cells

PROVIDER: PRJNA579932 | ENA |

REPOSITORIES: ENA

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Genome-wide analysis of histone marks in N1E115 cells

Project description:Neuronal nitric oxide synthase 1 (NOS1) produces the gaseous signaling molecule nitric oxide (NO), which plays important roles in the development and function of the nervous system. The regulation of Nos1 gene expression is incompletely understood. Here, we explored the role of physiological hypoxia in the control of Nos1 transcription and the underlying mechanisms using N1E115 mouse neuroblastoma cells as a model. N1E115 cells were cultured for 3 days at high (20%) and low (4%) oxygen levels. Following the verification of upregulation of Nos1 mRNA and protein levels in response to 4% oxygen, we analyzed the genome-wide distribution of the histone marks H3K27ac, H3K27me3, H3K36me3, H3K4me1, and H3K4me3 by chromatin immunoprecipitation-sequencing.

2024-02-14 | GSE139478 | GEO

Genome-wide analysis of POLR2, CTCF, RAD21, and H3K9ac binding in N1E115 cells

Project description:Neuronal nitric oxide synthase 1 (NOS1) produces the gaseous signaling molecule nitric oxide (NO), which plays important roles in the development and function of the nervous system. The regulation of Nos1 gene expression is incompletely understood. Here, we explored the role of physiological hypoxia in the control of Nos1 transcription and the underlying mechanisms using N1E115 mouse neuroblastoma cells as a model. N1E115 cells were cultured for 3 days at high (20%) and low (4%) oxygen levels. Following the verification of upregulation of Nos1 mRNA and protein levels in response to 4% oxygen, we analyzed the genome-wide distribution of CTCF, RAD21, total and S2- or S5-phosphorylated RNA polymerase II, and the histone mark H3K9ac by chromatin immunoprecipitation-sequencing.

2024-02-14 | GSE139481 | GEO

Genome-wide analysis of HIF1A and ARNT binding in N1E115 cells

Project description:Neuronal nitric oxide synthase 1 (NOS1) produces the gaseous signaling molecule nitric oxide (NO), which plays important roles in the development and function of the nervous system. The regulation of Nos1 gene expression is incompletely understood. Here, we explored the role of physiological hypoxia in the control of Nos1 transcription and the underlying mechanisms using N1E115 mouse neuroblastoma cells as a model. N1E115 cells were cultured for 3 days at high (20%) and low (4%) oxygen levels. Following the verification of upregulation of Nos1 mRNA and protein levels in response to 4% oxygen, we analyzed HIF1A and ARNT binding by chromatin immunoprecipitation-sequencing.

2024-02-14 | GSE139477 | GEO

Genome-wide analysis of HIF1A and ARNT binding in N1E115 cells

Project description:Genome-wide analysis of HIF1A and ARNT binding in N1E115 cells

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Genome wide ChIP-Seq of two histone marks in primary myoblasts

Project description:Genome wide ChIP-Seq of two histone marks in primary myoblasts

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Genome-wide maps of chromatin histone methylation marks in neonatal and adult cardiomyocytes

Project description:Epigenetic changes in DNA and chromatin are implicated in organogenesis and cell differentiation. Through a genome-wide chromatin-immunoprecipitation DNA-sequencing approach (ChIP-seq) we analyses the enrichment of H3K79me2 and H3K4me3 (histone methylation marks associated with transcriptional activation) and H3K27me3 and H3K9me3 (histone methylation marks associated with transcriptional repression) in neonatal and adult cardiomyocytes. The histone methylation profile obtained was correlated with an Illumina gene expression profile from the same samples. Our results demonstrate that histone methylation, and in particular the DOT1L-mediated H3K79me2 mark, drives cardiomyogenesis through the definition of a specific transcriptional landscape Profiling of H3K79me2, H3K4me3, H3K27me3 and H3K9me3 in neonatal and adult cardiomyocytes

2014-12-22 | E-GEOD-45174 | biostudies-arrayexpress

Genome-wide maps of chromatin histone methylation marks in neonatal and adult cardiomyocytes

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Genome-wide maps of histone marks, Sox2, Oct4 and PARP-1 in mES cells.

Project description:ChIP-seq was performed to analyze genome-wide distribution of PARP-1 and histone marks H3K4me3, H3K27me3 in mES cells, and examine the effect of PARP-1 knockout on Sox2 and Oct4 genome-wide distribution in ES cells.

2017-01-23 | GSE74112 | GEO

Determine the genome-wide localization of histone and histone marks in different stages of D. discoideum

Project description:Determine the genome-wide localization of histone and histone marks in different stages of D. discoideum

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Genome-wide analysis of POLR2, CTCF, RAD21, and H3K9ac binding in N1E115 cells

Project description:Genome-wide analysis of POLR2, CTCF, RAD21, and H3K9ac binding in N1E115 cells

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