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Escherichia coli O25b:H4-ST131

ABSTRACT: New KPC variant with a decreased susceptibility to ceftazidime-avibactam in the ST131-H30-Rx/C2 Escherichia coli high-risk clone

PROVIDER: PRJNA579968 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	WIRF01.dat.gz	Other
	WIRF01.fasta.gz	Fasta.gz
	WIRF01.master.dat	Other

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Escherichia coli O25b:H4-ST131

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Escherichia coli O25b:H4-ST131

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Escherichia coli O25b:H4-ST131

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Comprehensive Assessment of Initial Adaptation of ESBL Positive ST131 E. coli to Carbapenem Exposure

Project description:Background: It remains unclear how high-risk Escherichia coli lineages, like sequence type (ST) 131, initially adapt to carbapenem exposure in its progression to becoming carbapenem resistant. Methods: Carbapenem mutation frequency was measured in multiple subclades of extended-spectrum β-lactamase (ESBL) positive ST131 clinical isolates using a fluctuation assay followed by whole genome sequencing (WGS) characterization. Genomic, transcriptomic, and porin analyses of ST131 C2/H30Rx isolate, MB1860, under prolonged, increasing carbapenem exposure was performed using two distinct experimental evolutionary platforms to measure fast vs. slow adaptation. Results: All thirteen ESBL positive ST131 strains selected from a diverse (n=184) ST131 bacteremia cohort had detectable ertapenem (ETP) mutational frequencies with a statistically positive correlation between initial ESBL gene copy number and mutation frequency (r = 0.87, P<1e-5). WGS analysis of mutants showed initial response to ETP exposure resulted in significant increases in ESBL gene copy numbers or mutations in outer membrane porin (Omp) encoding genes in the absence of ESBL gene amplification with subclade specific adaptations. In both experimental evolutionary platforms, MB1860 responded to initial ETP exposure by increasing blaCTX-M-15 copy numbers via modular, insertion sequence 26 (IS26) mediated pseudocompound transposons (PCTns). Transposase activity driven by PCTn upregulation was a conserved expression signal in both experimental evolutionary platforms. Stable mutations in Omp encoding genes were detected only after prolonged increasing carbapenem exposure consistent with clinical observations. Conclusions: ESBL gene amplification is a conserved response to initial carbapenem exposure, especially within the high-risk ST131 C2 subclade. Targeting such amplification could assist with mitigating carbapenem resistance development.

2024-08-04 | GSE273456 | GEO

Escherichia coli O25b:H4-ST131 strain:EC958

Project description:Escherichia coli O25b:H4-ST131 strain:EC958 Epigenomics

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Escherichia coli O25b:H4-ST131 strain:EC958

Project description:Escherichia coli O25b:H4-ST131 strain:EC958 Genome sequencing

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Escherichia coli O25b:H4-ST131 strain:UR40

Project description:Escherichia coli O25b:H4-ST131 strain:UR40 Genome sequencing and assembly

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Escherichia coli O25b:H4-ST131 strain:EC958

Project description:The serum resistome of the globally disseminated, multidrug resistant uropathogenic E. coli ST131 strain EC958

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Novel Mechanisms of Antibiotic Resistance in Mismatch Repair-Deficient Pseudomonas aeruginosa hypermutators

Project description:Using in vitro directed evolution, we show that mismatch repair-deficient Pseudomonas aeruginosa can engage novel resistance mechanisms to ceftazidime-avibactam.

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