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Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease

ABSTRACT: Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease

PROVIDER: PRJNA579993 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10356200.fastq.gz	Fastqsanger.gz
	SRR10356201.fastq.gz	Fastqsanger.gz
	SRR10356202.fastq.gz	Fastqsanger.gz
	SRR10356203.fastq.gz	Fastqsanger.gz
	SRR10356204.fastq.gz	Fastqsanger.gz

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Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease

Project description:Purpose: Idenfication of microRNA biomarkers of Chronic wasting disease in serum from infected elk Methods: Illumina next generation was used to profile abundance of serum miRNA in elk naturally infected with chronic wasting disease and Hamsters experimentally infected with the 263K scrapie prion strains Results: A signature of 21 miRNAs with diagnostic potential was found to be altered in abundance in serum from CWD infected elk. Of these, 6 were similarily altered in the serum from the 263K infected hamsters Conclusion: These altered miRNA signatures may serve as the basis for non-invasive diagnostic assays for chronic wasting disease and may shed light on the pathogenesis of prion infection

2019-10-29 | GSE139501 | GEO

Circulating serum miRNAs as potential diagnostic biomarkers for oesophageal adenocarcinoma

Project description:Exosomes were purified from 250 ul serum using ExoQuickTm. The presence of particles consistent in size with exosomes (60-150nm) was confirmed using a Nanosight LM10. miRNA was extracted from exosomes using an miRNeasy Serum/Plasma kit (Qiagen, #217184). miRNA was reversed transcribed using a TaqMan® microRNA Reverse Transcription Kit (Life technologies, #4366596). miRNA profiling was performed with a high throughput TaqMan® OpenArray® Human microRNA panel (Life technologies, #4461104). The panel consisted of probes for 754 human miRNAs that are based on miRNA sequences derived from Sanger miRBase v14. MegaplexTM Primer Human Pool A v2.1 and Human Pool B v2.0 or v3.0 The poor prognosis and rising incidence of oesophageal adenocarcinoma highlight the need for improved methods for detection of this cancer. Molecular biomarkers offer potential for this. The potential for circulating miRNAs as biomarkers in some other cancers has been shown, but circulating miRNAs have not been well characterized in oesophageal adenocarcinoma. This study investigated whether circulating miRNAs could be used to detect oesophageal adenocarcinoma.

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CIRCULATING SERUM MICRO-RNA AS NON-INVASIVE DIAGNOSTIC BIOMARKERS OF ENDOMETRIOSIS

Project description:CIRCULATING SERUM MICRO-RNA AS NON-INVASIVE DIAGNOSTIC BIOMARKERS OF ENDOMETRIOSIS

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Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease.

Project description:Chronic wasting disease (CWD) is an emerging infectious prion disorder that is spreading rapidly in wild populations of cervids in North America. The risk of zoonotic transmission of CWD is as yet unclear but a high priority must be to minimize further spread of the disease. No simple diagnostic tests are available to detect CWD quickly or in live animals; therefore, easily accessible biomarkers may be useful in identifying infected animals. MicroRNAs (miRNAs) are a class of small, non-coding RNA molecules that circulate in blood and are promising biomarkers for several infectious diseases. In this study we used next-generation sequencing to characterize the serum miRNA profiles of 35 naturally infected elk that tested positive for CWD in addition to 35 elk that tested negative for CWD. A total of 21 miRNAs that are highly conserved amongst mammals were altered in abundance in sera, irrespective of hemolysis in the samples. A number of these miRNAs have previously been associated with prion diseases. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the discriminative potential of these miRNAs as biomarkers for the diagnosis of CWD. We also determined that a subgroup of 6 of these miRNAs were consistently altered in abundance in serum from hamsters experimentally infected with scrapie. This suggests that common miRNA candidate biomarkers could be selected for prion diseases in multiple species. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses pointed to a strong correlation for 3 of these miRNAs, miR-148a-3p, miR-186-5p, miR-30e-3p, with prion disease.

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Circulating serum microRNAs as potential diagnostic biomarkers of post-traumatic stress disorder: A pilot study

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Chromatin immunoprecipitation of human Hela cells grown under serum starved condtions to identify new targets genes for ETS-domain transcription factor Elk-1

Project description:Chromatin immunoprecipitation coupled with microarray analysis (ChIP-chip) was carried out to identify new target genes for ETS-domain transcription factor Elk-1. Experiment was done in Hela cells under serum starved conditions.

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Chromatin immunoprecipitation of human HeLa cells grown under serum starved conditions to identify new target genes for ETS-domain transcription factor Elk-1

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