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Analysis of LPS stimulation on F480low CD11b High Macrophages from WT and muMT KO mice

ABSTRACT: Analysis of LPS stimulation on F480low CD11b High Macrophages from WT and muMT KO mice

PROVIDER: PRJNA587154 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10386833_1.fastq.gz	Fastqsanger.gz
	SRR10386833_2.fastq.gz	Fastqsanger.gz
	SRR10386834_1.fastq.gz	Fastqsanger.gz
	SRR10386834_2.fastq.gz	Fastqsanger.gz
	SRR10386835_1.fastq.gz	Fastqsanger.gz

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Analysis of LPS stimulation on F480low CD11b High Macrophages from WT and muMT KO mice

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Analysis of LPS stimulation on F480Hi CD11blo Macrophages from WT and muMT KO mice

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Analysis of LPS stimulation on F480Low CD11b High Macrophages from WT and PI3KδE1020K-B KI mice

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Analysis of LPS stimulation on F480Hi CD11blo Macrophages from WT and muMT KO mice

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Expression data of CD117+ splenocytes from C57BL/6 WT and RasGRP4 KO mice upon LPS stimulation

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LPS time-course in WT and Stk40 KO BMDMs

Project description:Bone marrow was harvested from Rosa26CreER; Stk40+/+ (WT; n = 3) and Rosa26CreER; Stk40loxp/loxp (Stk40 KO; n = 3) mice and differentiated for 6 days in the presence of 100 nM 4-OHT to generate WT and Stk40 KO bone-marrow derived macrophages (BMDMs). 2. On day 7 following differentiation BMDMs were treated with 100 ng x ml-1 LPS and harvested at 0 hrs, 6 hrs, 16 hrs, and 32 hrs following LPS exposure. 3. The cells were snap-frozen at the time of harvest. RNA was extracted using the Qiagen RNeasy mini kit as per manufacturer’s protocol including the on-column DNase digestion. Groups: There are cells from 3 mice x 2 genotypes x 4 time points G1: WT 0 hr LPS G2: WT 6 hr LPS G3: WT 16 hr LPS G4: WT 32 hr LPS G5: Stk40 KO 0 hr LPS G6: Stk40 KO 6 hr LPS G7: Stk40 KO 16 hr LPS G8: Stk40 KO 32 hr LPS

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ChIP-seq to profile LPS-induced RNA polymerase II transcription in bone marrow-derived macrophages from WT and Hes1 KO mice

Project description:We characterized the RNA polymerase II occupancy on gene loci in WT and Hes1 KO BMDMs under untreated and LPS-stimulated conditions WT and Hes1 KO BMDMs were left untreated or stimulated with LPS for 1 hour. Pol II ChIP was performed and the DNA products were subject to ChIPseq

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