Zinc effect on mice
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA587484 | ENA |
REPOSITORIES: ENA
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Project description:This study underlines the beneficial use of excess dietary zinc in preventing colitis and inflammatory events. Zn-induced gene expression profiles were analyzed at ileum and colon level. A total number of 101 profiles were found differentially expressed during Zn supplemented diet, with an unbalanced pattern between these two tissues. A largest number of genes were found up-regulated in colon (22 genes), unlike the ileum (6 genes). At the other side, the number of down-regulated genes was higher in ileum (63 genes) against the colon (15 genes). Very few common profiles were detected in these two tissues, 3 up- and 2 down-regulated genes respectively. Genes coding for metallothioneins (Mt1, Mt2) and for phosphatidylinositol-4-phosphate 5-kinase (Pip5k1a) were found commonly up-regulated in ileum and colon, with the highest FC values. Genes coding for the zinc transporter of solute carrier family 39 (Slc39a4) and for lymphotoxin B (Ltb) were found commonly down-regulated with among the lowest FC values. Despite the different patterns, functional clustering of expressed genes converged for substantially the same physiological activities in both ileum and colon: intestinal tissue protection and epithelium reestablishment, immune system regulation as well as digestive and metabolic targeted functions.
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Project description:To identify the molecular pathways that are perturbed due to transient zinc chelation Zinc is known to regulate the functions of about 10% of the human proteome and a large number of physiological processes that are zinc dependent have been identified and characterized under conditions of zinc deficiency and supplementation. As zinc homeostasis is closely linked to the normal functioning of both prokaryotic and eukaryotic cells, many pathogens are directly or indirectly affected by perturbations in zinc homeostasis. Dengue virus (DENV), a mosquito-borne, positive-strand RNA virus from the family Flaviviridae, has emerged as one of the major public health concerns in India and recent estimates suggest that over 60 million people globally get infected with DENV every year. The crystal structures of NS5 protein of DENV and West Nile virus have identified zinc binding site in RdRp domain and propose an important structural role for zinc ions in polymerase activity. Therefore, we investigated whether perturbation in intracellular zinc pools influence dengue infection. We utilized N,N,N’,N’-tetrakis(2-pyridinylmethyl)-1,2-ethanediamine (TPEN), a zinc-specific chelator, to mimic zinc-deficiency in cell culture models of infection and investigated the effect of zinc depletion on DENV life-cycle.
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| MSV000088135 | MassIVE