Project description:The study was conducted on a model of Lactiplantibacillus plantarum, one of the most studied species widely used in the food industry as a probiotic microorganism and/or microbial starter culture. As a result of step-by-step selection from the L. plantarum 8p-a3 strain isolated from the «Lactobacterin» probiotic, the L. plantarum 8p-a3-Clr-Amx strain was obtained, showing increased resistance, compared with the parent strain, to amoxicillin-clavulanic acid (MIC 20 mcg/ml) and clarithromycin (MIC 10 mcg/ml). The L. plantarum strain DMC-S1 was isolated from the intestine of Drosophila melanogaster Canton-S line. Extracellular vesicles of this bacterium can play a significant role in the drug-resistance development and host-microbe interactions.
Project description:Poplar is a short-rotation woody crop routinely studied because of its importance as a sustainable bioenergy crop. The establishment of a successful poplar plantation partially depends on its rhizosphere, a dynamic zone governed by complex interactions between plant roots and a plethora of commensal, mutualistic, symbiotic, or pathogenic microbes that shape plant fitness. Here, we examined a consortium of ectomycorrhizal fungi and a beneficial Pseudomonas sp. strain GM41 for their effect on plant growth (height, stem girth, leaf, and root growth) and growth rate over time of four poplar genotypes of Populus trichocarpa. We also compare the total organic carbon and plant exometabolites profiles produced by these different poplar genotypes when colonized by the microbial consortium. We determined that when comparing the treatments to the control, plant growth parameters were not significantly different across the poplar genotypes eight weeks post-inoculation. However, total organic carbon and exometabolite profiles were significantly different between the genotypes and due to the treatments. These findings indicate that this microbial consortium can induce early and different signaling responses in poplar.
