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Real-time quantitative PCR analysis of OC-C and CH under osteoarthritic condition

ABSTRACT: Real-time quantitative PCR analysis of OC-C and CH under osteoarthritic condition

PROVIDER: PRJNA589303 | ENA |

REPOSITORIES: ENA

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Real-time quantitative PCR analysis of OC-C and CH under osteoarthritic condition

Project description:IL-1β (1 ng/ml) was introduced via medium feed to the cartilage side to induce OA-like phenotype in both OC and CH tissue chip. The catabolic, inflammatory and chondrogenic gene expression were compared between these two groups to assess the effect of osseous tissue on chondral tissue under OA condition.

2019-11-14 | GSE140335 | GEO

Real-time quantitative PCR analysis of phenotype of osteochondral tissue chip

Project description:In this study, we aimed to develop microphysiological osteochondral (OC) tissue chips derived from human induced pluripotent stem cells (iPSCs) to model the pathologies of OA. We first induced iPSCs into mesenchymal progenitor cells (iMPCs) and optimized the chondro- and osteo-inductive conditions for iMPCs. Then iMPCs were encapsulated into photocrosslinked gelatin scaffolds and cultured within a dual-flow bioreactor, in which the top stream was chondrogenic medium and the bottom stream was osteogenic medium. After 28 days of differentiation, biphasic OC tissue and monophasic chondral (CH) tissue chips were successfully generated and phenotypes were confirmed by real time RT-PCR. The OC tissues were cut into Top and Bottom (Bot), and both parts were compared with each other. CH tissue were compared with their phenotype on Day 0. Total RNA was extracted from the samples and processed for qPCR according to the manufacturer's instructions.

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Real-time quantitative PCR analysis of celecoxib treatment on OC tissue chip

Project description:To test the utility of iPSC-derived OC tissue chip in drug screening, Celecoxib, a selective cyclooxygenase 2 (COX-2) inhibitor commonly used to treat OA, was administered to the system. Celecoxib was administered at 10 µM concentration, and showed no noticeable adverse effect on the health of normal chondral tissue. Administration of Celecoxib to both OC-C and OC-B were used to simulate the typically systemic action of Celecoxib, represented by the “SY” group. In addition, we also examined the potential effect of intraarticular (IA) application of Celecoxib in treating OA, by delivery only to OC-C, namely the “IA” group. Normal OC tissues without any treatments served as the control (CL).

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Real-time quantitative PCR analysis of celecoxib treatment on OC tissue chip

Project description:Real-time quantitative PCR analysis of celecoxib treatment on OC tissue chip

| PRJNA589302 | ENA

Real-time quantitative PCR analysis of chondrosupportive activity of bone component in the engineered osteochondral tissue

Project description:To explore the influence of subchondral bone on the health of cartilage under normal physiology conditions, we compared the phenotype of OC-C with CH in terms of chondrogenic and hypertrophic gene expression

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Real-time quantitative PCR analysis of UPR genes in human normal and osteoarthritic chondrocytes

Project description:Human chondrocytes were obtained from the cartilage of femoral condyles and tibial plateaus from individuals within 12 hours of death (normal) and from patients undergoing total knee arthroplasty (osteoarthritic, OA). The chondrocytes were released from the cartilage, seeded at high density, and cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum and an antibiotic mixture (100 units/ml penicillin base and 100 μg/ml streptomycin base) at 37°C in a humidified atmosphere of 5% CO2/95% air. Total RNA was extracted from the primary chondrocytes and processed for qPCR according to the manufacturer's instructions (SA Biosciences RT2 Profiler PCR Array PAHS-089Z).

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Expression data from human OC+BAP+ double positive versus OC-BAP- double negative cells

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Real-time quantitative PCR analysis of UPR genes in human osteoarthritic chondrocytes specimens treated with thapsigargin compared to the untreated (control) chondrocytes

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Real-time quantitative PCR analysis of UPR genes in human osteoarthritic chondrocytes specimens treated with tunicamycin compared to the untreated (control) chondrocytes

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Expression data from human OC+BAP+ double positive versus OC-BAP- double negative cells

2013-05-17 | E-GEOD-47060 | biostudies-arrayexpress