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Loss of major nutrient sensing and signaling pathways suppresses starvation lethality in electron transport chain mutants

ABSTRACT: Loss of major nutrient sensing and signaling pathways suppresses starvation lethality in electron transport chain mutants

PROVIDER: PRJNA589319 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10441000_1.fastq.gz	Fastqsanger.gz
	SRR10441000_2.fastq.gz	Fastqsanger.gz
	SRR10441001_1.fastq.gz	Fastqsanger.gz
	SRR10441001_2.fastq.gz	Fastqsanger.gz
	SRR10441002_1.fastq.gz	Fastqsanger.gz

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Loss of major nutrient sensing and signaling pathways suppresses starvation lethality in electron transport chain mutants (DenseTimeCourse)

Project description:Here we sought to comprehensively characterize the role of the electron transport chain in yeast models of aging. Using a panel of ETC mutants, we observed that ETC mutants fail to survive starvation when grown to saturation in standard SD growth medium. This starvation lethality is associated with a significantly lower cytosolic pH and dysregulated gene expression compared to wild type cells. In an unbiased genetic suppressor screen, we found that loss of function mutations in major cellular nutrient sensing/signaling pathways (Ras/PKA, Tor, PP2A) along with a number of gene expression regulators can prevent starvation lethality in these mutants. Together our results suggest that the ETC plays a critical regulatory role in mounting an appropriate response to starvation through communication with major nutrient sensing/signaling pathways in the cell.

2019-11-14 | GSE140353 | GEO

Loss of major nutrient sensing and signaling pathways suppresses starvation lethality in electron transport chain mutants (ETC_Mutants)

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Loss of major nutrient sensing and signaling pathways suppresses starvation lethality in electron transport chain mutants (DenseTimeCourse)

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Loss of major nutrient sensing and signaling pathways suppresses starvation lethality in electron transport chain mutants (ETC_Mutants)

Project description:Loss of major nutrient sensing and signaling pathways suppresses starvation lethality in electron transport chain mutants (ETC_Mutants)

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Loss of major nutrient sensing and signaling pathways suppresses starvation lethality in electron transport chain mutants

Project description:This SuperSeries is composed of the SubSeries listed below.

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Loss of major nutrient sensing and signaling pathways suppresses starvation lethality in electron transport chain mutants.

Project description:The electron transport chain (ETC) is a well-studied and highly conserved metabolic pathway that produces ATP through generation of a proton gradient across the inner mitochondrial membrane coupled to oxidative phosphorylation. ETC mutations are associated with a wide array of human disease conditions and to aging-related phenotypes in a number of different organisms. In this study, we sought to better understand the role of the ETC in aging using a yeast model. A panel of ETC mutant strains that fail to survive starvation was used to isolate suppressor mutants that survive. These suppressors tend to fall into major nutrient sensing and signaling pathways, suggesting that the ETC is involved in proper starvation signaling to these pathways in yeast. These suppressors also partially restore ETC-associated gene expression and pH homeostasis defects, though it remains unclear whether these phenotypes directly cause the suppression or are simply effects. This work further highlights the complex cellular network connections between metabolic pathways and signaling events in the cell and their potential roles in aging and age-related diseases.

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Project description:Although tumor growth requires the mitochondrial electron transport chain (ETC), the relative contribution of Complex I (CI) and II (CII), the gatekeepers for initiating electron flow, remains unclear. Here, we report that loss of CII, but not CI, reduces melanoma tumor growth by increasing antigen presentation and T cell-mediated killing. This is driven by succinate-mediated transcriptional and epigenetic activation of major histocompatibility complex I-antigen processing and presentation (MHC-APP) genes that is independent of interferon signaling. Furthermore, knock-out of MCJ, to promote electron entry preferentially via CI, provides proof-of-concept of ETC rewiring to achieve anti-tumor responses without side effects associated with an overall reduction in mitochondrial respiration in non-cancer cells. Our results hold therapeutic potential for tumors that have reduced MHC-APP expression, a common mechanism of cancer immunoevasion.

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