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Dichotomous Engagement of HDAC3 Activity Governs Inflammatory Responses [GRO-Seq]

ABSTRACT: Dichotomous Engagement of HDAC3 Activity Governs Inflammatory Responses [GRO-Seq]

PROVIDER: PRJNA590194 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10486671.fastq.gz	Fastqsanger.gz
	SRR10486672.fastq.gz	Fastqsanger.gz
	SRR10486673.fastq.gz	Fastqsanger.gz
	SRR10486674.fastq.gz	Fastqsanger.gz
	SRR10486675.fastq.gz	Fastqsanger.gz

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Dichotomous Engagement of HDAC3 Activity Governs Inflammatory Responses

Project description:Dichotomous Engagement of HDAC3 Activity Governs Inflammatory Responses

| PRJNA590241 | ENA

Dichotomous Engagement of HDAC3 Activity Governs Inflammatory Responses [GRO-Seq]

Project description:Histone deacetylase 3 (HDAC3) is unique among the HDAC superfamily of chromatin modifiers that silence transcription through enzymatic modification of histones, because interaction with nuclear receptor corepressors (NCoR1/2) is required for engagement of its catalytic activity. However, loss of HDAC3 also represses transcription. Here we report that, during lipopolysaccharide (LPS) activation of macrophages, the deacetylase activity of HDAC3 is selectively engaged at ATF3-bound enhancers that repress anti-inflammatory genes. By contrast, LPS-stimulated recruitment of HDAC3 to ATF2-bound sites without NCoR1/2 activates pro-inflammatory genes by a non-canonical mechanism whereby catalytically inactive HDAC3 stably interacts with p65. Consistent with this bimodal inflammatory modulation, deletion of HDAC3 in macrophages safeguards mice from lethal exposure to LPS, but this protection is not conferred by genetic or pharmacological abolition of HDAC3 catalytic activity. Thus, HDAC3 is a dichotomous transcriptional activator and repressor whose deacetylase-independent functions are critical in priming the innate immune system.

2020-04-30 | GSE140573 | GEO

Dichotomous Engagement of HDAC3 Activity Governs Inflammatory Responses [RNA-Seq]

Project description:Dichotomous Engagement of HDAC3 Activity Governs Inflammatory Responses [RNA-Seq]

| PRJNA590243 | ENA

Dichotomous Engagement of HDAC3 Activity Governs Inflammatory Responses [ChIP-Seq]

Project description:Dichotomous Engagement of HDAC3 Activity Governs Inflammatory Responses [ChIP-Seq]

| PRJNA590208 | ENA

Dichotomous Engagement of HDAC3 Activity Governs Inflammatory Responses [RNA-Seq]

2020-04-30 | GSE140610 | GEO

Dichotomous Engagement of HDAC3 Activity Governs Inflammatory Responses [ChIP-Seq]

2020-04-30 | GSE140581 | GEO

Dichotomous engagement of HDAC3 activity governs inflammatory responses.

Project description:The histone deacetylases (HDACs) are a superfamily of chromatin-modifying enzymes that silence transcription through the modification of histones. Among them, HDAC3 is unique in that interaction with nuclear receptor corepressors 1 and 2 (NCoR1/2) is required to engage its catalytic activity1-3. However, global loss of HDAC3 also results in the repression of transcription, the mechanism of which is currently unclear4-8. Here we report that, during the activation of macrophages by lipopolysaccharides, HDAC3 is recruited to activating transcription factor 2 (ATF2)-bound sites without NCoR1/2 and activates the expression of inflammatory genes through a non-canonical mechanism. By contrast, the deacetylase activity of HDAC3 is selectively engaged at ATF3-bound sites that suppress Toll-like receptor signalling. Loss of HDAC3 in macrophages safeguards mice from lethal exposure to lipopolysaccharides, but this protection is not conferred upon genetic or pharmacological abolition of the catalytic activity of HDAC3. Our findings show that HDAC3 is a dichotomous transcriptional activator and repressor, with a non-canonical deacetylase-independent function that is vital for the innate immune system.

| S-EPMC7725280 | biostudies-literature

Dichotomous Engagement of HDAC3 Activity Governs Inflammatory Responses

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-04-30 | GSE140611 | GEO

Circadian Epigenomic Control of Skeletal Muscle Fuel Metabolism by HDAC3

Project description:We address the function of HDAC3 in skeletal muscle metabolism We performed HDAC3 ChIP-seq, RNA-seq, and GRO-seq in mouse muscles at different times of the day and compared between WT and HDAC3-depleted muscles.

2016-09-06 | E-GEOD-79696 | biostudies-arrayexpress

Gene expression profiling of HDAC3-depleted mouse liver rescued with wild-type exogenous HDAC3

Project description:We have shown that intravenous injection of HDAC3 floxed mice with adeno-associated virus (AAV) expressing Cre depletes hepatic HDAC3, upregulates lipogenic gene expression, and causes fatty liver. When AAV-Flag-HDAC3 wild-type (WT) is co-injected along with AAV-Cre, the exogenous HDAC3 is expressed at endogenous levels and can completely rescue fatty liver phenotype. Here we profile transcriptome of the rescued WT livers in comparison with HDAC3-depleted (KO) livers. 4-months old C57BL/6 male mice were co-injected with AAV-Cre or AAV-Cre plus AAV-Flag-HDAC3. Mice were fed ad libitum and harvested at 5 pm (ZT10) at 2-weeks post-injection. Liver total RNA was extracted and hybridized to Affymetrix Mouse Gene 1.0ST array.

2013-11-25 | E-GEOD-49386 | biostudies-arrayexpress

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