ABSTRACT: Variation in global transcriptomic response to Perkinsus marinus infection among Eastern oyster families highlights potential mechanisms of disease resistance
Project description:Perkinsus marinus is an intracellular parasitic protozoan that is responsible for serious disease epizootics in marine bivalve molluscs worldwide and along with P. olseni belongs to the OIE list of notified diseases. Despite all available information on P. marinus genomics, more baseline data is required at the proteomic level for a better understanding of P. marinus biological processes, including virulence mechanisms. In the present study, we have established in vitro clonal cultures of P. marinus from infected gills and mantle tissues of C. rhizophorae to evaluate the parasite cellular proteomic profile. A high throughput label-free shotgun HDMS approach using nanoUPLC-MS was used. Our intention was to provide the first comprehensive proteome profile of P. marinus that might serve as a valuable resource for future investigations involving comparative analyses of P. marinus from different regions, as well as comparisons of different species of Perkinsus.
2018-01-08 | PXD003727 | Pride
Project description:epigenetic and transcriptomic responses to Perkinsus marinus infection in the eastern oyster Crassostrea virginica.
| PRJNA604121 | ENA
Project description:Survival and transcriptomic responses to different Perkinsus marinus exposure methods in an Eastern oyster family
| PRJNA691949 | ENA
Project description:oyster hemolymph transcriptomes challenged with Perkinsus marinus and apoptosis modulators
Project description:ESTs in which transcription levels in oyster gill differed among sampling times after heat shock and between families with high or low survival of heat shock were identified through expression profiling of 1,675 spot pairs Keywords: comparison of families characterized by low or high survival after heat shock Oyster families were characterized by survival after heat shock, exposed to a sub-lethal heat shock, and gill was collected before and at 1, 3, 6, and 24 h afterwards. Differences among sampling times and between family types were assessed using a 2-way analysis of variance. In total, 1,675 ESTs were included in the analysis.
