Similar Datasets
Project description:The species-specific identification of fibre origin is essential in archaeology but reveals challenging for closely related species. This is particularly true between the four South American Camelids (SAC) species: alpaca, guanaco, llama and vicuña. The analysis of proteins extracted from hairs and/or yarns by proteomics has emerged as a powerful method to differentiate between species. However, for SAC, the database information available is very poor, which limits this approach. In this study, we analysed 42 modern and 4 archaeological reference samples from the four SAC species.
2020-11-09 | PXD018980 | Pride
Project description:South American camelids (SACs) play an important role in Andean economies, but reproductive inefficiencies, especially early embryonic losses, present significant challenges to herd productivity. This study provides the first proteomic analysis of preimplantation alpaca embryos, offering insights into the molecular mechanisms underlying embryo development and implantation. Using mass spectrometry, we identified 510 proteins in 15-day post-mating (dpm) alpaca embryos. Functional enrichment analysis revealed significant pathways related to cytoskeletal organization, metabolism, embryo elongation, and implantation. Key proteins associated with conceptus elongation, maternal recognition of pregnancy, and steroidogenesis were detected, including keratins (KRT8, KRT18), actin (ACTG1), annexin A2 (ANXA2), and enzymes involved in estradiol and cortisol biosynthesis. Additionally, proteins involved in prostaglandin synthesis, including PTGS2, were identified, supporting their role in conceptus elongation and implantation. This pioneering study provides a foundational dataset for understanding alpaca embryo development at the proteomic level, opening new paths for improving reproductive technologies in SACs. The findings highlight the molecular processes driving alpaca embryogenesis and provide a basis for future research on optimizing assisted reproductive technologies in camelids.
2025-09-05 | PXD061991 | Pride
Project description:Leaf colour variation is observed in several plants. We obtained two types of branches with yellow (H1) and variegated (H2) leaves from Camellia sinensis. To reveal the mechanisms that underlie the leaf colour variations, proteomic analysis using label-free MS-based approach was performed using leaves from variants and normal branches (CKs).
2018-10-26 | PXD004750 | Pride
Project description:A gene expression profiling study on two major cotton species that are cultivated for fibre, Gossypium hirsutum (L.) and Gossypium barbadense (L.), at different stages during fibre development using a printed cDNA microarray was undertaken to identify potential candidate genes for manipulation to improve fibre quality. Keywords: Species comparison, development
2009-07-21 | GSE10363 | GEO
Project description:Seed coat colour is determined by the type of pigment deposited in the seed coat cells. It is related to important agronomic traits of seeds, such as seed dormancy, longevity, oil content, protein content and fibre content. In Brassica napus, inheritance of seed coat colour is related to maternal effects and pollen effects (xenia effects). In this research, we isolated a mutation of yellow seeded B. napus controlled by a single Mendelian locus with pollen effect. Microcopy of transverse sections of the mature seed shows pigment is deposited only in the epidermal cells, the first cell layer of seed coat. By Illumina Hiseq 2000 sequencing technology, a total of 12 G clean data, 116x coverage of coding sequences of B. napus, was achieved from 26-day old brown and yellow seeds. It was assembled into 172,238 independent transcripts and 55,637 unigenes by Trinity. A total of 150 orthologous genes of Arabidopsis transparent testa (TT) genes were mapped in silico to 19 chromosomes of B. napus. Only 49 of the TT orthologous genes are transcripted in seeds. However transcription of all the orthologs was independent of the embryonal control of seed coat colour. Of all the Trinity-assembled unigenes, only 55 genes were found to be differentially expressed between the brown seeds and yellow mutant. Among them 50 were up-regulated and 5 were down-regulated in the yellow seeds as compared to the brown counterpart. By KEGG classification, 14 metabolic pathways were enriched significantly. Of these, 5 pathways: phenylpropanoid biosynthesis, cyanoamino acid metabolism, plant hormone signal transduction, metabolic pathways and biosynthesis of secondary metabolites, were related with seed coat pigmentation. Free amino acid quantification showed that Ala and Phe were produced at higher levels in the embryo of yellow seeds as compared to brown seeds. This increase was not observed in the seed coat. Moreover, the excess amount of free Ala was exactly twice that of Phe in the 26-day embryo of yellow seeds. Pigment indispensable substrate chalcone is synthesized from two molecules of Ala and one molecule of Phe. The correlation between accumulation of Ala and Phe and disappearance of pigment in the yellow seeded mutant indicate that embryonal control of seed coat colour is related with Phe and Ala metabolism in the embryo of B. napus.
2018-05-19 | GSE69137 | GEO