Project description:Fruits of sea buckthorn have abundant antioxidants and attractive colors. They provide excellent materials to study the relationships among fruit color, antioxidant and fruit quality
Project description:Sea buckthorn (Hippophae rhamnoides L.) is well adapted to extreme temperatures ranging from −43 to 40°C and is ideal material to study mechanisms related to low-temperature stress tolerance
Project description:Sea buckthorn (Hippophae rhamnoides L.) is an economically important shrub or small tree indigenous to Asia and Europe. The most well-recognized medicinal and nutraceutical products derive from the berry oil, which is rich in essential fatty acids, monounsaturated palmitoleic acid (16:1 ω-7), linoleic (18:2 ω-6) and alpha-linolenic (18:3 ω-3) acids. In this study, tandem mass tags (TMT) MS/MS were used to investigate protein profiles of lipid metabolism in sea buckthorn berries harvested at 30, 50 and 70 days after flowering. A total of 8626 proteins were detected and 6170 of these were quantified. Further analyses of the proteins and their pathways revealed initiation of fatty acid accumulation throughout the whole berry development. Activity of most of key enzymes, which are related to biosynthesis pathways of triacylglycerol and fatty acids, had peaked at 50 days; but triacylglycerol synthesis through the PDAT (phospholipid: diacylglycerol acyltransferase) catalytic pathway of occurred mostly at the early stage of berry development. Protein expression patterns related to lipid accumulation were also verified at the transcript level using quantitative real-time polymerase chain reaction. The proteome profiles determined here will improve our understanding of the process of lipid accumulation and the dynamic changes in metabolic pathways during sea buckthorn berry development.
Project description:Most of Colorectal cancer (CRC) diagnosed are candidates for surgical resection with curative intent, although colorectal surgery is associated with some complications that could be life-threatening. Antibiotic prophylaxis is commonly used prior to the admission for the prevention of postoperative complications. However, this intervention can change the composition of intestinal microbiota and promote adverse inflammatory outcomes in CRC patients after surgery.
It seems the combination of different fungal extracts could be beneficial because of their role in gut microbiota modulation and their anti-inflammatory activity. Therefore, the fungal extract nutraceutical MICODIGEST 2.0 could be used to reduced the complications after CRC surgery.
Based on this hypothesis, we have designed a double-bind randomized clinical trial to evaluate the effect of MICODIGEST 2.0 on the complications after surgery with curative intent for CRC.
