Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Fetal monocytes possess increased metabolic capacity and replace primitive macrophages in tissue macrophage development

ABSTRACT: Fetal monocytes possess increased metabolic capacity and replace primitive macrophages in tissue macrophage development

PROVIDER: PRJNA590368 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Fetal monocytes possess increased metabolic capacity and replace primitive macrophages in tissue macrophage development

Project description:We studied alveolar macrophage (AM) development after single and competitive transplantation of different precursors from YS, fetal liver, and lung into neonatal Csf2ra-/- mice, which lack endogenous AM. Fetal monocytes, promoted by Myb, outcompeted primitive MΦ (pMΦ) in empty AM niches and preferentially developed to mature AM, which is associated with enhanced mitochondrial respiratory and glycolytic capacity and repression of the transcription factors c-Maf and MafB. Interestingly, AM derived from pMΦ failed to efficiently clear alveolar proteinosis and protect from fatal lung failure following influenza virus infection. Thus, our data demonstrate superior developmental and functional capacity of fetal monocytes over pMΦ in AM development and underlying mechanisms explaining replacement of pMΦ in fetal tissues.

2019-12-30 | GSE140645 | GEO

Fetal monocytes possess increased metabolic capacity and replace primitive macrophages in tissue macrophage development.

Project description:Tissue-resident macrophages (MΦTR ) originate from at least two distinct waves of erythro-myeloid progenitors (EMP) arising in the yolk sac (YS) at E7.5 and E8.5 with the latter going through a liver monocyte intermediate. The relative potential of these precursors in determining development and functional capacity of MΦTR remains unclear. Here, we studied development of alveolar macrophages (AM) after single and competitive transplantation of different precursors from YS, fetal liver, and fetal lung into neonatal Csf2ra-/- mice, which lack endogenous AM. Fetal monocytes, promoted by Myb, outcompeted primitive MΦ (pMΦ) in empty AM niches and preferentially developed to mature AM, which is associated with enhanced mitochondrial respiratory and glycolytic capacity and repression of the transcription factors c-Maf and MafB. Interestingly, AM derived from pMΦ failed to efficiently clear alveolar proteinosis and protect from fatal lung failure following influenza virus infection. Thus, our data demonstrate superior developmental and functional capacity of fetal monocytes over pMΦ in AM development and underlying mechanisms explaining replacement of pMΦ in fetal tissues.

| S-EPMC6996567 | biostudies-literature

Organic fertilizer replace

Project description:Organic fertilizer replace Raw sequence reads

| PRJNA735545 | ENA

Colorectal cancer cells possess an equipotent capacity to enter a reversible diapause-like state to survive chemotherapy

Project description:Colorectal cancer cells possess an equipotent capacity to enter a reversible diapause-like state to survive chemotherapy

| PRJNA606811 | ENA

Engineering transcriptional regulators using REPLACE

Project description:Engineering transcriptional regulators using REPLACE

| PRJNA1133800 | ENA

Colorectal cancer cells possess an equipotent capacity to enter a reversible diapause-like state to survive chemotherapy

Project description:Cancer cells enter a reversible drug tolerant persister (DTP) state to evade death from chemotherapies and targeted agents. It is increasingly appreciated that DTPs are an important driver of therapy failure and tumor relapse. We combined cellular barcoding and mathematical modeling in patient-derived colorectal cancer models to identify and characterize DTPs in response to chemotherapy. Barcode analysis revealed no loss in clonal complexity of tumors that entered the DTP state and recurred following treatment cessation. Our data fits a mathematical model where all cancer cells, and not a small subpopulation, possess an equipotent capacity to become DTPs. Mechanistically, we determined that DTPs display remarkable transcriptional and functional similarities to diapause, a reversible state of suspended embryonic development triggered by unfavorable environmental conditions. Our study provides insight into how cancer cells use a developmentally conserved mechanism to drive the DTP state pointing to novel therapeutic opportunities to target DTPs.

2021-01-07 | GSE145356 | GEO

Engineering transcriptional regulators using REPLACE

Project description:Transcriptional regulators are fundamental elements in synthetic biology. We sought to use REPLACE to evolve synthetic transcriptional regulators. Through weeks of directed evolution experiments, we succesufuly modified the sensitivity of TetR and PadR to ligand molecules.

2024-09-30 | GSE271879 | GEO

Blue‐shifting green fluorescent proteins using REPLACE

Project description:Blue‐shifting green fluorescent proteins using REPLACE

| PRJNA1133788 | ENA

Human Naïve Epiblast Cells Possess Unrestricted Lineage Potential

Project description:Human Naïve Epiblast Cells Possess Unrestricted Lineage Potential

| PRJNA700707 | ENA

Human Naïve Epiblast Cells Possess Unrestricted Lineage Potential

Project description:Human Naïve Epiblast Cells Possess Unrestricted Lineage Potential

| PRJNA700867 | ENA