Project description:DNA deamination occurs constantly in a cell and causes DNA damage. As this damage can be deleterious, organisms have evolved many systems to eliminate it. Deamination of cytosine, guanine, adenine, and 5-methylcytosine results in the formation of uracil, xanthine, hypoxanthine, and thymine, respectively. Sodium bisulfite is a kind of DNA deaminating agent that can increase the frequency of DNA deamination in cells. This study measures the transcriptome profile of Haloferax volcanii H26 strain and HVO_RS06830 gene knockout strain, induced with different concentrations of sodium bisulfite.
Project description:Purpose: The Haloferax volcanii ∆tfeB strain provided a unique opportunity to study a putative role of TFEβ in the regulation of gene expression. Results: The deletion of the tfeB gene in H. volcanii results in the aberrant expression of approximately one third of all genes, consistent with its function as a basal transcription initiation factor. Interestingly, tfeB deletion particularly affects foreign genes including a prophage region. Conclusions: Our results confirm the dual function of TFE as basal factor and regulator of transcription
Project description:Identification of global gene expression profiling when Haloferax volcanii H26 strain is grown in minimal medium supplemented with two nitrogen sources, 10 mM L-alanine and 10 mM ammonium chloride.
