Project description:To investigate the mechanism of annual rhythms in Japanese cedar, annual time series samples were collected from the cuttings planted in natural condition. Also, to investigate the effects of photoperiod and temperature during transition to dormancy, the samples of cuttings grown in the controlled-environmental chamber were analyzed by a microarray.
Project description:<p>Organic carbon in seawater plays a significant role in the global carbon cycle. The concentration and composition of dissolved organic carbon, operationally defined in this project as organic carbon that passes through a 0.2 µm filter, reflect the actions of the biological community and chemical reactions that occur in seawater. Here, we repeatedly sampled the oligotrophic northwest Sargasso Sea in the vicinity of the Bermuda Atlantic Time-Series Study site (BATS) to quantitatively follow select known compounds within the pool of dissolved organic matter in the upper 1000 meters of the water column over a four-year period. Metabolite concentrations revealed patterns with depth and time with most metabolites showing surface enrichment and lower concentrations with depth. Select metabolites had a pattern of increased and decreased concentrations throughout the year, which was observed in each of the years sampled. Vitamins, including pantothenic acid, biotin, and riboflavin, presented annual increases in the winter period when mixed layer depths are deepest. Light-sensitive riboflavin also showed significant decreases during daylight hours under diel sampling. The metabolites examined in this study are all components of central carbon metabolism. By examining these metabolites at finer resolution and in a relatively long time series, we have clues on microbial actions in marine systems, data which are fundamental to understanding the chemical response of marine systems to future changes in climate.</p>
Project description:Rising atmospheric CO2 concentrations are leading to ocean acidification, altering the inorganic carbon buffer system with consequences for marine organisms. Here we applied RNA-seq and iTRAQ quantification to investigate the potential impacts of ocean acidification on the temperate coastal marine diatom Skeletonema marinoi.
Project description:This SuperSeries is composed of the following subset Series: GSE22915: Mussel (Mytilus galloprovincialis) digestive gland tissue: gene expression profiles across an annual cycle GSE23049: Mytilus galloprovincialis: development of female gonads GSE23050: Mytilus galloprovincialis: development of male gonads GSE23051: Mytilus galloprovincialis: differences between male and female gene expression patterns in gonads (mantle tissue) Refer to individual Series