Project description:We performed high-throughput RNA sequencing to characterize possible differences in the transcriptome of primary human aortic Vascular Smooth Muscle cells abrogated of NEIL3 mRNA via antisense oligonucleotides targeting NEIL3 exon 4, compared with control cells treated with a scramble version of the same antisense oligonucleotide
Project description:We performed high-throughput RNA sequencing to characterize possible differences in the transcriptome of murine aortic Vascular Smooth Muscle cells from both 26 weeks-old ApoE knockout and ApoE/Neil3 double knockout mice fed a regular diet.
Project description:Analysis of differential gene expression of aortic vascular smooth muscle cells isolated from Tie2floxed:SM22α Cre- (WT) and Tie2floxed:SM22α Cre+ (KO) mice Looking for candidates, that could potentialy be upregulated or downregulated
Project description:The impact EGFR, AT1R and TP receptor individual or simultaneous activations on the gene expression was investigated in mouse aortic vascular smooth muscle cells (aVSMC)
Project description:Transcription factor FoxM1 is expressed in proliferating cells, and its expression is critical for cell proliferation in embryos and tumors. FoxM1 regulates a multi-gene transcriptional network for cell cycle regulation. We used microarrays to detail the global program of gene expression either directly or indirectly regulated by FoxM1, and distinct classes of up- and down-regulated genes. Experiment Overall Design: Human aortic vascular smooth muscle cells (HAVSMC) were transfected with FoxM1 siRNA and control siRNA for 48 hours. Total RNA was extracted, purified, and prepared for hybridization on Affymetrix microarrays.