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RNA-seq of NIH3T3 cells treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

ABSTRACT: RNA-seq of NIH3T3 cells treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

PROVIDER: PRJNA591160 | ENA |

REPOSITORIES: ENA

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RNA-seq of NIH3T3 cells treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

Project description:To identify gene expression changes associated with treatment of EVs from MDA-MB-231 [231] and MCF10A [10A] cells) in NIH3T3 cells, we analyzed RNA isolated from PBS- or EV-treated NIH3T3 cells. Gene expression in NIH3T3 cells treated with EV from MDA-MB-231 cells was compared to cells treated with PBS or EV from MCF10A cells, both of which served as controls in this experiment.

2020-05-01 | GSE140852 | GEO

RNA-seq of liver tissue from mice treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

Project description:To identify gene expression changes in liver associated with circulating EV that carry high levels of miR-122 (from MDA-MB-231 and MCF10A/miR-122 cells), we analyzed RNA isolated from the liver of PBS- or EV-treated female NOD scid gamma (NSG) mice. Mice had received tail-vein injected EV (or PBS) twice a week for 5 weeks (~10 ug EV per injection). Gene expression in liver from mice treated with EV from MDA-MB-231 or MCF10A/miR-122 cells was compared to cells treated with PBS or EV from MCF10A cells, both of which served as controls in this experiment.

2021-02-01 | GSE152391 | GEO

RNA-seq of cancer-associated fibroblasts (CAF) treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

Project description:To identify gene expression changes associated with treatment of EV that carry high levels of miR-105 (from MDA-MB-231 and MCF10A/miR-105 cells) in human breast tumor derived CAF, we analyzed RNA isolated from PBS- or EV-treated CAF. Gene expression in CAF treated with EV from MDA-MB-231 or MCF10A/miR-105 cells was compared to cells treated with PBS or EV from MCF10A cells, both of which served as controls in this experiment.

2018-02-11 | GSE106503 | GEO

RNA-seq of liver tissue from mice treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

Project description:RNA-seq of liver tissue from mice treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

| PRJNA639156 | ENA

RNA-seq of cancer-associated fibroblasts (CAF) treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

Project description:RNA-seq of cancer-associated fibroblasts (CAF) treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

| PRJNA417064 | ENA

Cancer-cell-secreted miR-9-5p regulates cholesterol homeostasis to promote tumor metastasis [PBS, MCF-10A EV and MDA-MB-231 EV]

Project description:To investigate the effects of breast cancer derived EVs on liver metabolism, NSG mice had received 5 weeks of i.v. injections of MCF-10A EVs, MDA-MB-231 EVs or PBS. We then performed gene expression profiling analysis using data obtained from RNA-seq of liver from mice received 5 weeks of i.v. injections of MCF-10A EVs, MDA-MB-231 EVs or PBS.

2024-08-31 | GSE222622 | GEO

Cancer-cell-secreted miR-9-5p regulates cholesterol homeostasis to promote tumor metastasis [PBS, MCF-10A EV and MDA-MB-231 EV]

Project description:Cancer-cell-secreted miR-9-5p regulates cholesterol homeostasis to promote tumor metastasis [PBS, MCF-10A EV and MDA-MB-231 EV]

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Differential response of MDA‑MB‑231 breast cancer and MCF10A normal breast cells to cytoskeletal disruption.

Project description:Metastasis remains a major clinical problem in cancer diagnosis and treatment. Metastasis is the leading cause of cancer‑related mortality but is still poorly understood. Cytoskeletal proteins are considered potential therapeutic targets for metastatic cancer cells because the cytoskeleton serves a key role in the migration and invasion of these cells. Vimentin and F‑actin exhibit several functional similarities and undergo quantitative and structural changes during carcinogenesis. The present study investigated the effects of vimentin and F‑actin deficiency on the survival and motility of breast cancer cells. In metastatic breast cancer cells (MDA‑MB‑231) and breast epithelial cells (MCF10A), vimentin was knocked down by small interfering RNA and F‑actin was depolymerized by latrunculin A, respectively. The effect of reduced vimentin and F‑actin content on cell viability was analyzed using the MTT assay and the proliferative capacity was compared by analyzing the recovery rate. The effect on motility was analyzed based on two processes: The distance traveled by tracking the cell nucleus and the movement of the protrusions. The effects on cell elasticity were measured using atomic force microscopy. Separately reducing vimentin or F‑actin did not effectively inhibit the growth and motility of MDA‑MB‑231 cells; however, when both vimentin and F‑actin were simultaneously deficient, MDA‑MB‑231 cells growth and migration were severely impaired. Vimentin deficiency in MDA‑MB‑231 cells was compensated by an increase in F‑actin polymerization, but no complementary action of vimentin on the decrease in F‑actin was observed. In MCF10A cells, no complementary interaction was observed for both vimentin and F‑actin.

| S-EPMC10565893 | biostudies-literature

RNA-seq of MCF10A transduced with an inducible lentiviral MYB expression system

Project description:The non-tumourigenic breast cell line MCF10A was transduced using pINDUCER21-MYB vector to express MYB upon addition of doxyclyclin (DOX), and compared to an empty vector (EV) control (pINDUCER21 (ORF-EG)) with and without the addition of DOX

2023-12-01 | E-MTAB-12977 | biostudies-arrayexpress

RNA-seq of gastrocnemius (GA) muscle tissue from mice treated with extracellular vesicles (EV) from MCF-10A or MDA-MB-231 cells

Project description:To compare gene expression changes in skeletal muscle caused by EVs from normal (MCF-10A) and cancer (MDA-MB-231) cells, we analyzed RNA isolated from the GA muscle of EV-treated female NOD scid gamma (NSG) mice. Mice had received tail-vein injections of EVs twice a week for 5 weeks (~10 ug EV per injection). Gene expression in muscle from mice treated with MDA-MB-231-derived EVs was compared to mice treated with MCF-10A-derived EVs.

2022-01-30 | GSE156909 | GEO

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