Project description:DBA/2N Ahsg -/- mice suffer from sever soft tissue calcification. Gene expression in brown adipose tissue isolated from the pelvic region of 6 week old mice was analyzed, representing early stage calcification. Evaluation of the gene expression pattern was performed to provide insights into the mechanisms associated with soft tissue calcification.
Project description:SIRT1 is a NAD+-dependent protein deacetylase. SIRT1 plays key roles in metabolic regulation and adaptation. In this study, we examined the difference of gene expression in brown adipose tissue from WT and SIRT1tg mice. SIRT1 transgenic model (heterozygous transgenic model) has already been described (Pfluger et al., 2008). Here we used homozygote transgenic mice which had been backcrossed to C57Bl/6N background. 3 months old WT and SIRT1tg mice were fed with a low fat diet. After sacrifice, total mRNA obtained from brown adipose were used for microarray.
Project description:SIRT1 is a NAD+-dependent protein deacetylase. SIRT1 plays key roles in metabolic regulation and adaptation. In this study, we examined the difference of gene expression in brown adipose tissue from WT and SIRT1tg mice.
Project description:Gene expression in brown adipose tissue and in primary adipocytes derived thereof from PKGI-/- mice was studied in comparison to wild type mice.
Project description:DBA/2N Ahsg -/- mice suffer from sever soft tissue calcification. Gene expression in whole kidney of 5-6 week old mice were analyzed, at this stage calcification is not yet macroscopically detectable. Evaluation of the gene expression pattern was performed to provide insights into the mechanisms preceding the formation of mineralized lesions.
Project description:Gene expression profile from brown adipose tissues of Prdm16 knockout and wile type mice. Prdm16 is a transcription factor that regulates the thermogenic gene program in brown and beige adipocytes. However, whether Prdm16 is required for the development or physiological function of brown adipose tissue (BAT) in vivo has been unclear. By analyzing mice that selectively lacked Prdm16 in the brown adipose lineage, we found that Prdm16 was dispensable for embryonic BAT development.
Project description:Analysis of brown adipose tissue (BAT) isolated from wildtype (WT) and liver kinase B1 (LKB1) deletion mice (Ad/LKB1). Results provide insight into molecular mechanisms underlying paralysis of Ad/LKB1 mice