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Differential tissue-specific damage caused by bacterial epididymo-orchitis in the mouse.

ABSTRACT: Differential tissue-specific damage caused by bacterial epididymo-orchitis in the mouse.

PROVIDER: PRJNA591923 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10539857.fastq.gz	Fastqsanger.gz
	SRR10539858.fastq.gz	Fastqsanger.gz
	SRR10539859.fastq.gz	Fastqsanger.gz
	SRR10539860.fastq.gz	Fastqsanger.gz
	SRR10539861.fastq.gz	Fastqsanger.gz

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Differential tissue-specific damage caused by bacterial epididymo-orchitis in the mouse.

Project description:This study investigated the differential responses of the epididymis and testis to infection with uropathogenic Escherichia coli (UPEC) in adult mice. In contrast to the cauda epididymidis, which exhibited ongoing damage following UPEC-infection, spermatogenic disruption was reversible and the caput epididymidis was largely unaffected. The caput epididymidis is structurally and functionally very different from the cauda, whereas beta-defensins as important epididymal antimicrobial factors are highly expressed in both tissues. The transcriptome of the testis, caput, corpus and cauda epididymidis were analysed in normal, wildtype C57BL/6J mice. Distinct differences in gene signatures were observed between caput and cauda epididymidis, particularly among immune-related genes, whereas corpus and cauda were much more alike. Beta-defensins were found at high levels in both caput and cauda epididymidis, whereas other antimicrobial factors, such as Lipocalin 2 and Lypd8 were found expressed selectively high in the caput epididymidis and hence may play an important role in local protection from bacterial pathogens, including UPEC.

2020-05-06 | GSE141071 | GEO

Differential tissue-specific damage caused by bacterial epididymo-orchitis in the mouse.

Project description:Ascending bacterial urinary tract infections can cause epididymo-orchitis. In the cauda epididymidis, this frequently leads to persistent tissue damage. Less coherent data is available concerning the functional consequences of epididymo-orchitis on testis and caput epididymidis. This in vivo study addresses the functional and spatial differences in responsiveness of murine epididymis and testis to infection with uropathogenic Escherichia coli (UPEC). Whole transcriptome analysis (WTA) was performed on testis, caput, corpus and cauda epididymidis of adult C57BL/6 J wildtype mice. Following UPEC-induced epididymo-orchitis in these mice, epididymal and testicular tissue damage was evaluated histologically and semi-quantitatively at 10 days and 31 days post-inoculation. Expression of inflammatory markers and candidate antimicrobial genes were analysed by RT-qPCR. WTA revealed distinct differences in gene signatures between caput and cauda epididymidis, particularly amonst immunity-related genes. Cellular and molecular signs of testicular inflammation and disruption of spermatogenesis were noticed at day 10, but recovery was observed by day 31. In contrast to the cauda, the caput epididymidis did not reveal any signs of gross morphological damage or presence of pro-inflammatory processes despite confirmed infection. In contrast to beta-defensins, known UPEC-associated antimicrobial peptides (AMP), like Lcn2, Camp and Lypd8, were inherently highly expressed or upregulated in the caput following infection, potentially allowing an early luminal protection from UPEC. At the time points investigated, the caput epididymidis was protected from any obvious infection/inflammation-derived tissue damage. Studies addressing earlier time-points will conclude whether in the caput epididymidis a pro-inflammatory response is indeed not essential for effective protection from UPEC.

| S-EPMC7187874 | biostudies-literature

Differential expression caused by Dicer knockout in small intestine

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2024-02-20 | GSE206778 | GEO

miRNA profile of duck duodenum mucosa damage caused by transportation stress

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Inhibition of IL-6 signaling attenuates vascular damage caused by HIF1α activation

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Serious leaf damage caused by Osslac7 mutation arouses self-protection mechanism in rice

Project description:OsSLAC7 in Oryza sativa L. is a homolog of Arabidopsis thaliana AtSLAC1, which contains conserved C4-Dicarboxylate transporter domain. Loss of its function caused cell membrane instability and serious leaf damage. We used Affymetrix Rice Genome Array to detail the differences in global gene expression between wildtype and the OsSLAC7 T-DNA insertion mutants, and to make clear the bioprocesses affected by the mutation. OsSLAC7(Os01g0385400) T-DNA insertion mutant and wildtype japonica rice cultivar Zhonghua11 were used for RNA extraction and hybridization on Affymetrix Rice Genome Array.

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