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H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line

ABSTRACT: H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line

PROVIDER: PRJNA592544 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10560100_1.fastq.gz	Fastqsanger.gz
	SRR10560100_2.fastq.gz	Fastqsanger.gz
	SRR10560101_1.fastq.gz	Fastqsanger.gz
	SRR10560101_2.fastq.gz	Fastqsanger.gz
	SRR10560102_1.fastq.gz	Fastqsanger.gz

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H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [ChIP-seq]

Project description:H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [ChIP-seq]

| PRJNA592545 | ENA

H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [RNA-seq]

Project description:H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [RNA-seq]

| PRJNA592546 | ENA

H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [RNA-seq D4]

Project description:H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [RNA-seq D4]

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H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [ChIP-seq]

Project description:We used Ultra-Low native ChIP to interrogate the H3K27me3 and H3K9me3 chromatin states in primordial germ cells before and after gonadal reprogramming. Our data demonstrate that repressive heterochromatin is remodelled during epigenetic reprogramming.

2021-09-27 | GSE141180 | GEO

H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [RNA-seq]

Project description:To investigate the role of EZH2 in transcription regulation in mouse primordial germ cells, we isolated Ctrl and Ezh2 CKO male and female PGCs at E13.5. Our data demonstrates that EZH2-mediated H3K27me3 is required for regulating the expression of meiotic genes in the female germ cells. Furthermore, RNA-seq analysis shows that EZH2 is essential for transcriptional repression of retrotransposons.

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H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [RNA-seq D4]

Project description:To investigate the role of EZH2 in transcription regulation in mouse primordial germ cell like cells (PGCLCs), we isolated Ctrl and Ezh2 KO PGCLCs. Our data demonstrates that EZH2-mediated H3K27me3 is dispensible for transcription regulation in PGCLCs.

2021-09-27 | GSE163375 | GEO

Heterochromatin Protein 1γ Regulates Epigenetic Reprogramming in Primordial Germ Cells

Project description:Temporal and spatial regulation of trimethylation of histone H3 lysine27 (H3K27me3) is crucial for cell lineage commitment and development. Resetting the ground state of H3K27me3 is important for establishing a proper development program of primordial germ cells (PGCs). The mechanisms that regulate global erasure of epigenetic information in the germ line are not well understood. Here we show that HP1γ suppresses the demethylation activity of Utx through direct interaction in vitro and in vivo. Down-regulation of mammalian heterochromatin protein 1 gamma (HP1γ) coincides with transient loss of H3K27me3 in PGCs on Embryonic Day 10.5 (E10.5). Furthermore, transient abolishment of HP1γ promotes the induction of embryonic stem cells into putative PGCs in vitro. These data reveal a cross-talk between HP1γ and Utx in controlling histone H3K27 methylation status, thereby define HP1γ as a molecular regulator of germ cell epigenetic reprogramming.

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The H3K27 demethylase Utx facilitates somatic and germ cell epigenetic reprogramming to pluripotency [ChIP-Seq]

Project description:Pluripotency can be induced in somatic cells by ectopic expression of defined transcription factors, however the identity of epigenetic regulators driving the progression of cellular reprogramming requires further investigation. Here we uncover a non-redundant role for the JmjC-domain-containing protein histone H3 methylated Lys 27 (H3K27) demethylase Utx, as a critical regulator for the induction, but not for the maintenance, of primed and naM-CM-/ve pluripotency in mice and in humans. Utx depletion results in aberrant H3K27me3 repressive chromatin demethylation dynamics, which subsequently hampers the reactivation of pluripotency promoting genes during reprogramming. Remarkably, Utx deficient primordial germ cells (PGCs) display a cell autonomous aberrant epigenetic reprogramming in vivo during their embryonic maturation, resulting in the lack of functional contribution to the germ-line lineage. H3K27me3 and H3K4me3 were measured genome-wide in the following cell types: Utx+/Y (WT) and Utx-/Y (KO) mouse ES cells and mouse embryonic fibroblast (MEF) before and after DOX induction (initiating reprogramming by OSKM factors).

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The H3K27 demethylase Utx facilitates somatic and germ cell epigenetic reprogramming to pluripotency [Affymetrix gene expression]

Project description:Pluripotency can be induced in somatic cells by ectopic expression of defined transcription factors, however the identity of epigenetic regulators driving the progression of cellular reprogramming requires further investigation. Here we uncover a non-redundant role for the JmjC-domain-containing protein histone H3 methylated Lys 27 (H3K27) demethylase Utx, as a critical regulator for the induction, but not for the maintenance, of primed and naM-CM-/ve pluripotency in mice and in humans. Utx depletion results in aberrant H3K27me3 repressive chromatin demethylation dynamics, which subsequently hampers the reactivation of pluripotency promoting genes during reprogramming. Remarkably, Utx deficient primordial germ cells (PGCs) display a cell autonomous aberrant epigenetic reprogramming in vivo during their embryonic maturation, resulting in the lack of functional contribution to the germ-line lineage. Samples include UTX+/Y (WT) and UTX-/Y (KO) cells from mouse ES cells and mouse embryonic fibroblast (MEF) before and after DOX induction (initiating reprogramming by OSKM factors). One sample is OSKM-induced Nanog-/- fibroblasts.

2012-05-29 | E-GEOD-35775 | biostudies-arrayexpress

H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-09-27 | GSE141182 | GEO