Project description:Blochmannia endosymbionts (Gammaproteobacteria) live in bacteriocytes, which are specialized cells found in the genus Camponotus and its neighbor genera. In this announcement, we describe the complete genome sequence of the Blochmannia endosymbiont of Colobopsis nipponica, which originated from a colony collected in the Republic of Korea.
Project description:The transcriptome of Blochmannia floridanus, the endosymbiont of the carpenter ant Camponotus floridanus, is presented during eight developmental stages of its holometabolous host by use of a whole genome DNA-macroarray. The detected transcription patterns indicate the presence of local transcription units as well as global regulatory mechanisms. Yet, the overall regulation scale is very modest, rarely exceeding a factor of three which is in line with the low number of transcriptional regulators present in this reduced genome. A large number of genes show differential expression in different life stages and a distinct expression pattern of genes possibly involved in symbiotic function as compared to housekeeping genes is apparent. However, these transcriptional changes are small as compared to the changes in the number of bacteria during host development which is highest in pupae and in young imagines. Control of replication of the bacteria in certain life stages may therefore be the decisive parameter influencing overall gene expression of Blochmannia in the animal. The few highly expressed genes like those encoding molecular chaperones exhibit a significantly higher G+C-content than moderately expressed genes. Keywords: Host stage-dependent gene expression
Project description:Blochmannia endosymbionts, belonging to Gammaproteobacteria, live in bacteriocytes, which are specialized cells for these bacterial species in the Camponotus genus (carpenter ants). In this announcement, we describe the complete genome sequence of the Blochmannia endosymbiont of Camponotus nipponensis, which originated from a C. nipponensis colony collected in the Republic of Korea.