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An Ancient COI1-independent Function for Reactive Electrophilic Oxylipins in Thermotolerance [array]

ABSTRACT: An Ancient COI1-independent Function for Reactive Electrophilic Oxylipins in Thermotolerance [array]

PROVIDER: PRJNA593448 | ENA |

REPOSITORIES: ENA

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An Ancient COI1-independent Function for Reactive Electrophilic Oxylipins in Thermotolerance

Project description:An Ancient COI1-independent Function for Reactive Electrophilic Oxylipins in Thermotolerance

| PRJNA593432 | ENA

An Ancient COI1-independent Function for Reactive Electrophilic Oxylipins in Thermotolerance [seq]

Project description:An Ancient COI1-independent Function for Reactive Electrophilic Oxylipins in Thermotolerance [seq]

| PRJNA593392 | ENA

An Ancient COI1-independent Function for Reactive Electrophilic Oxylipins in Thermotolerance [array]

Project description:Seeds of the double mutant opr3-3 coi1-1 were germinated in Johnson media supplemented with 0.5 µM coronatine, to select homozygous coi1-1 plants, at 21°C under long-day conditions (16-h light/8-h dark)

2020-03-04 | GSE141429 | GEO

An Ancient COI1-independent Function for Reactive Electrophilic Oxylipins in Thermotolerance

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-03-04 | GSE141430 | GEO

An Ancient COI1-independent Function for Reactive Electrophilic Oxylipins in Thermotolerance [seq]

Project description:Algal discs were transferred to a 12-well plate containing 2 ml liquid 0.5 Gamborg’s B5 mock or OPDA 25 µM, incubated for 2 hours on a shaker (120 rpm) and then the algae were frozen in liquid nitrogen and ground to a fine powder.

2020-03-04 | GSE141417 | GEO

TGA transcription factors and jasmonate-independent COI1 signalling regulate specific plant responses to reactive oxylipins.

Project description:Jasmonates and phytoprostanes are oxylipins that regulate stress responses and diverse physiological and developmental processes. 12-Oxo-phytodienoic acid (OPDA) and phytoprostanes are structurally related electrophilic cyclopentenones, which activate similar gene expression profiles that are for the most part different from the action of the cyclopentanone jasmonic acid (JA) and its biologically active amino acid conjugates. Whereas JA-isoleucine signals through binding to COI1, the bZIP transcription factors TGA2, TGA5, and TGA6 are involved in regulation of gene expression in response to phytoprostanes. Here root growth inhibition and target gene expression were compared after treatment with JA, OPDA, or phytoprostanes in mutants of the COI1/MYC2 pathway and in different TGA factor mutants. Inhibition of root growth by phytoprostanes was dependent on COI1 but independent of jasmonate biosynthesis. In contrast, phytoprostane-responsive gene expression was strongly dependent on TGA2, TGA5, and TGA6, but not dependent on COI1, MYC2, TGA1, and TGA4. Different mutant and overexpressing lines were used to determine individual contributions of TGA factors to cyclopentenone-responsive gene expression. Whereas OPDA-induced expression of the cytochrome P450 gene CYP81D11 was primarily regulated by TGA2 and TGA5, the glutathione S-transferase gene GST25 and the OPDA reductase gene OPR1 were regulated by TGA5 and TGA6, but less so by TGA2. These results support the model that phytoprostanes and OPDA regulate differently (i) growth responses, which are COI1 dependent but jasmonate independent; and (ii) lipid stress responses, which are strongly dependent on TGA2, TGA5, and TGA6. Identification of molecular components in cyclopentenone signalling provides an insight into novel oxylipin signal transduction pathways.

| S-EPMC3580818 | biostudies-literature

Reactive electrophilic oxylipins trigger a heat stress-like response through HSFA1 transcription factors.

Project description:Abiotic and biotic stresses are often characterized by an induction of reactive electrophile species (RES) such as the jasmonate 12-oxo-phytodienoic acid (OPDA) or the structurally related phytoprostanes. Previously, RES oxylipins have been shown massively to induce heat-shock-response (HSR) genes including HSP101 chaperones. Moreover, jasmonates have been reported to play a role in basal thermotolerance. We show that representative HSR marker genes are strongly induced by RES oxylipins through the four master regulator transcription factors HSFA1a, b, d, and e essential for short-term adaptation to heat stress in Arabidopsis. When compared with Arabidopsis seedlings treated at the optimal acclimation temperature of 37 °C, the exogenous application of RES oxylipins at 20 °C induced a much weaker induction of HSP101 at both the gene and protein expression levels which, however, was not sufficient to confer short-term acquired thermotolerance. Moreover, jasmonate-deficient mutant lines displayed a wild-type-like HSR and were not compromised in acquiring thermotolerance. Hence, the OPDA- and RES oxylipin-induced HSR is not sufficient to protect seedlings from severe heat stress but may help plants to cope better with stresses associated with protein unfolding by inducing a battery of chaperones in the absence of heat.

| S-EPMC5100025 | biostudies-literature

The coi1-2 mutant has a differential transcriptional program in response to Al stress

Project description:To investigate how JA regulates Al-induced inhibition of root growth, a transcriptional analysis through RNAseq was performed by comparing the coi1-2 mutant and WT plants in the presence or absence of Al. In the absence of Al, 149 and 147 genes were up- and down-regulated, respectively, at least 2-fold in the roots of the coi1-2 mutant and WT plants. In the presence of Al, 1747 and 5838 genes were up- and down-regulated, respectively, in the roots of WT plants, while 1449 and 3773 genes were up- and down-regulated, respectively in the coi1-2 mutant. While the comparison of the Al-exposed coi1-2 mutant and WT plants reveals that totally 1187 genes were up-regulated and only 197 genes were down-regulated at least 2-fold.

2017-03-07 | GSE83361 | GEO

Thermotolerance in B. subtilis

Project description:During thermotolerance development, B. subtilis cells can be primed by a short mild heat shock to survive a subsequent severe, otherwise lethal, heat stress. We wanted to assess the influence of transcriptional regulation during thermotolerance development in B. subtilis. We prepared total RNA of four differently treated samples of B. subtilis cells. For this purpose, exponential B. subtilis cells grown at 37 °C were divided and exposed for additional 15 min to temperatures of 37 °C, 48 °C, 53 °C or 15 min 48 °C followed by 15 min at 53 °C.

2014-05-01 | GSE45972 | GEO

Determination of Pseudomonas aeruginosa transcriptome induced by oleic acid or its derived oxylipins 10-HOME and 7,10-DiHOME.

Project description:Oxygenated unsaturated fatty acids, known as oxylipins, are signaling molecules commonly used for cell-to-cell communication in eukaryotes. However, a role for oxylipins in mediating communication in prokaryotes has not previously been described. Bacteria mainly communicate via quorum sensing (QS) , which involves the production and detection of diverse small molecules termed autoinducers. We showed that oleic acid-derived oxylipins 10-HOME and 7,10-DiHOME produced by Pseudomonas aeruginosa function as autoinducers of a novel quorum sensing system termed Oxylipin-Dependent QS Sytem (ODS). This experiment was designed to determine the genes whose expression is altered by these P. aeruginosa oxylipins. We found that the ODS system controls the cell density-dependent expression of a P. aeruginosa gene subset through the mediation of 10-HOME and 7,10-DiHOME oxylipins.

2018-12-05 | GSE123356 | GEO

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