Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Pervasive promoter-proximal regulation in archaea: RNA-seq of cells during exponential growth

ABSTRACT: Pervasive promoter-proximal regulation in archaea: RNA-seq of cells during exponential growth

PROVIDER: PRJNA593550 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10587775_1.fastq.gz	Fastqsanger.gz
	SRR10587775_2.fastq.gz	Fastqsanger.gz
	SRR10587776_1.fastq.gz	Fastqsanger.gz
	SRR10587776_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Pervasive promoter-proximal regulation in archaea: ChIP-exo exponential growth

Project description:Pervasive promoter-proximal regulation in archaea: ChIP-exo exponential growth

| PRJNA593009 | ENA

Pervasive promoter-proximal regulation in archaea: ChIP-seq exponential growth

Project description:Pervasive promoter-proximal regulation in archaea: ChIP-seq exponential growth

| PRJNA593012 | ENA

Pervasive promoter-proximal regulation in archaea: Permanganate ChIP-seq exponential growth

Project description:Pervasive promoter-proximal regulation in archaea: Permanganate ChIP-seq exponential growth

| PRJNA593214 | ENA

Pervasive promoter-proximal regulation in archaea

Project description:Pervasive promoter-proximal regulation in archaea

| PRJNA593004 | ENA

Pervasive promoter-proximal regulation in archaea: ChIP-seq exponential growth

Project description:Recruitment of RNA polymerase and initiation factors to the promoter is the only known mechanisms for transcription activation and repression in archaea. Whether any of the subsequent steps towards productive transcription elongation is involved in regulation is not known. We characterised how the basal transcription machinery is distributed along genes in the archaeon Sulfolobus solfataricus. We discovered a distinct early elongation phase where RNA polymerases sequentially recruit the elongation factors Spt4/5 and Elf1 to form the transcription elongation complex (TEC) before the TEC escapes into productive transcription. TEC escape is rate-limiting for transcription output during exponential growth. Oxidative stress causes changes in TEC escape that correlate with changes in the transcriptome. Our results thus establish that TEC escape contributes to the basal promoter strength and facilitates transcription regulation. Impaired TEC escape coincides with the accumulation of initiation factors at the promoter and recruitment of termination factor aCPSF1 to the early TEC. This suggests two possible mechanisms for how TEC escape limits transcription, physically blocking upstream RNA polymerases during transcription initiation and premature termination of early TECs.

2020-09-14 | GSE141286 | GEO

Pervasive promoter-proximal regulation in archaea: ChIP-exo exponential growth

2020-09-14 | GSE141289 | GEO

Pervasive promoter-proximal regulation in archaea: Permanganate ChIP-seq exponential growth

2020-09-14 | GSE141358 | GEO

Pervasive promoter-proximal regulation in archaea: RNA-seq of cells during exponential growth (Cappable _sRNA-seq)

Project description:Pervasive promoter-proximal regulation in archaea: RNA-seq of cells during exponential growth (Cappable _sRNA-seq)

| PRJNA655913 | ENA

Pervasive promoter-proximal regulation in archaea: ChIP-seq stationary phase

Project description:Pervasive promoter-proximal regulation in archaea: ChIP-seq stationary phase

| PRJNA594053 | ENA