Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome wide-binding profile of NRF2 in A549 NSCLC cells [ChIP-seq]

ABSTRACT: Genome wide-binding profile of NRF2 in A549 NSCLC cells [ChIP-seq]

PROVIDER: PRJNA593605 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10588628.fastq.gz	Fastqsanger.gz
	SRR10588629.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Genome wide-binding profile of NRF2 in A549 NSCLC cells [ChIP-seq]

Project description:This study aimed to elucidate the relationships between NRF2 and disease progression and provide insight into NRF2-mediated cancer progression/tumorigenesis by identifying novel genes and pathways regulated by NRF2 in A549 NSCLC cells

2019-12-05 | GSE141497 | GEO

Expression data from KEAP1 overexpression and NRF2 siRNA knockdown of A549 NSCLC cell

Project description:KEAP1 overexpressed and NRF2 siRNA knockdown A549 NSCLC cells were used to identify downstream genes of NRF2 pathway separately and by combinatorial analysis. We used triplicate microarrays of transfected A549 cells with mKeap1-GFP for overexpression, siRNAs targeting NRF2 for knockdown and siGFP as control respectively. As a result, we identified several genes which are involved in cancer metabolic functions in these cells. We used microarrays to identify the gene downregulated in both KEAP1 overexpressed and NRF2 siRNA knockdown A549 NSCLC cells and found a subset of downregulated genes which are involved in metabolic functions.

2017-08-02 | GSE94393 | GEO

Genome-wide maps of Nrf2 and Pol2 binding in A549 cells

Project description:Genome-wide maps of Nrf2 and Pol2 binding in A549 cells

| PRJNA451354 | ENA

Identification of Nrf2-regulated genes in A549 lung cancer cells by oligonucleotide microarray

Project description:To elucidate the mechanisms by which Nrf2 regulates cell growth, we performed global gene expression profiling of A549 lung cancer cells with knockdown of Nrf2. Gene networks associated with carbohydrate metabolism and drug metabolism were significantly downregulated in Nrf2-depleted A549 cells. Gene Set Enrichment Analysis revealed significant enrichment of genes associated with carbohydrate catabolic processes, positive regulation of metabolic processes, PPP, and arachidonic acid metabolism. In summary, this analysis revealed that Nrf2 positively regulates transcription of genes that play key roles in central carbon metabolism. A549 cells were transfected with non targeting NS siRNA or siRNA targeting Nrf2. Mock transfected A549 cells were treated with transfection reagent alone. We had 3 biological replicates for each of the 3 groups. Ninty six hours post transfection, cells were lysed and total RNA was isolated.

2013-07-04 | E-GEOD-38332 | biostudies-arrayexpress

Comparison of gene expression profiles of A549 cell treated with NRF2 siRNA and control siRNA

Project description:To examine the role of NRF2 in accelerating cell proliferation and to identify the target genes responsible for this function, transcriptome analysis was performed using A549 cells, in which NRF2 is constitutively activated. NRF2 was knocked down by siRNA against NRF2, and the gene expression profile was compared with that of A549 cells treated with control siRNA. To exclude off-target effects, three different siRNAs against NRF2 was independently applied. NRF2 siRNA or control siRNA was transfected into A549 cells. Cells were harvested 24 hours after transfection, and total RNA was purified.

2011-04-01 | E-GEOD-28230 | biostudies-arrayexpress

Nrf2 regulated genes in A549 cells

Project description:Nrf2 regulated genes in A549 cells

| PRJNA451429 | ENA

Genome wide screen of CREB and CRTC2 occupancy in LKB1 mutant NSCLC cell line A549

Project description:CREB and CRTC2 ChIPseq analysis in LKB1-deficient and LKB1-reconstituted A549 NSCLC cells

2019-07-26 | GSE128871 | GEO