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Pseudomonas aeruginosa strain:CMC-115

ABSTRACT: Pseudomonas aeruginosa strain:CMC-115 Genome sequencing and assembly

PROVIDER: PRJNA593770 | ENA |

REPOSITORIES: ENA

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Pseudomonas aeruginosa strain:CMC-097

Project description:Pseudomonas aeruginosa strain:CMC-097 Genome sequencing and assembly

| PRJNA660482 | ENA

Comparative transcriptomic analysis of Pseudomonas aeruginosa isolates from ICU patients with acute and chronic pneumonia

Project description:In this study, a comparative analysis was performed on two clinical Pa strains from pneumonia patients, 1) an acute strain (CMC-115) and 2) a chronic multidrug- and carbapenem-resistant strain (CMC-097), with distinct phenotypic and genetic characteristics. A transcriptome analysis using both the Pseudomonas DNA GeneChip microarray and Illumina RNA-Seq technologies was carried out for both strains at early-stationary growth phase in laboratory culture. The comparative transcriptomic analysis identified 134 genes differentially expressed ≥4.0-fold by both technologies between the two strains, which included virulence genes such as flagellar, pyoverdine, and phenazine genes, that were higher in the acute strain, CMC-115, and type 3 secretory system (T3SS) and pyochelin biosynthesis genes, that were higher in the chronic strain, CMC-097. In particular, the DNA microarrays suggested and the RNA-Seq analysis confirms the type 3 secretory system genes were completely missing from the acute strain, CMC-115. The combined analysis using the RNA-Seq and DNA microarray methods also identified important genes that were either missing or highly divergent from the Reference PAO1 genome used for the DNA microarray design. For example, the RNA-Seq data identified an operon containing several multidrug resistance genes: aacA27, beta-lactamase (blaOXA-2), qacΔE, sul1, and GNAT-N, in the chronic strain, CMC-097.

2021-07-23 | GSE180499 | GEO

Zinc acquisition in Pseudomonas aeruginosa

Project description:This study addresses the impact of zinc limitation on the opportunistic human pathogen, Pseudomonas aeruginosa. Zinc limitation was assessed in the P. aeruginosa PAO1 strain using an isogenic deletion mutant lacking the periplasmic, zinc solute-binding protein, znuA (PA5498). ZnuA delivers bound zinc to its cognate ABC transporter, ZnuBC, for import into the cytoplasm. Our transcriptional analyses revealed P. aeruginosa to possess a multitude of zinc acquisition mechanisms, each of which were highly up-regulated in the zinc-deficient znuA mutant strain. P. aeruginosa also utilized zinc-independent paralogues of zinc-dependent genes to maintain cellular function under zinc limitation. Together, these data reveal the complex transcriptional response and versatility of P. aeruginosa to zinc depletion.

2017-01-01 | GSE60177 | GEO

SigX ECF sigma factor deletion mutant expression profile in Pseudomonas aeruginosa in M9 minimal medium (M9G)

Project description:Analysis of a SigX knockout mutant of Pseudomonas aeruginosa H103 strain in minimal medium with glucose as carbon source (M9G). SigX, one of the 19 extra-cytoplasmic function sigma factors of P. aeruginosa, was only known to be involved in transcription of the gene encoding the major outer membrane protein OprF in Pseudomonas aeruginosa. Deletion of the ECF sigma factor sigX gene provide insights into the SigX role in several virulence and biofilm- related phenotypes in Pseudomonas aeruginosa.

2014-01-01 | GSE51076 | GEO

SigX ECF sigma factor deletion mutant expression profile in Pseudomonas aeruginosa H103 in LB medium

Project description:Analysis of a SigX knockout mutant of Pseudomonas aeruginosa H103 strain in LB.

2018-12-01 | GSE117438 | GEO

Biological characterization of virulent therapeutic bacteriophage PAK_P3 infecting Pseudomonas aeruginosa.

Project description:We have isolated and characterized several bacteriophages infecting Pseudomonas aeruginosa distantly related to Felix O1 virus and proposed they form a new subfamily named Felixounavirinae. The infectious cycle of bacteriophages belonging to this subfamily has not been studied yet in terms of gene expression. The present study reports the RNA-Seq analysis of bacteriophage PAK_P3 infecting PAK strain of P. aeruginosa. RNA profile of Host and Phage at 0min, 3.5min and 13 min after infection of Pseudomonas aeruginosa PAK strain with the Pseudomonas phage PAK P3. Three biological replicates for each time point.

2016-07-20 | E-GEOD-76513 | biostudies-arrayexpress

Transcription profiling by array of Pseudomonas aeruginosa wild type, BphP and BphO strains.

Project description:The transcriptome of two different Pseudomonas aeruginosa mutant strains were compared to the Pseudomonas aeruginosa wild type strain in the stationary growth phase

2010-04-24 | E-MEXP-2606 | biostudies-arrayexpress

Responses of Pseudomonas aeruginosa to low oxygen

Project description:In order to understand how Pseudomonas aeruginosa responds to low oxygen we grew strain PAO1 with 3 different oxygen concentrations: 2%, 0.4% and 0% supplemented with nitrate as an electron acceptor. Gene expression under these conditions was compared to that of cells grown with 20% oxygen. Keywords: Comparison of transcriptome profiles

2007-05-17 | GSE6741 | GEO

Comparative transcriptomic profiling of overexpression of PsrA in Pseudomonas aeruginosa PAO1

Project description:PsrA, a transcription factor belonging to the TetR family, is known to participate in the regulation of fatty acid metabolism, type III secretion system, and quinolone signaling in Pseudomonas aeruginosa. Using a psrA overexpression strain, this study conducted a transcriptomic analysis to examine the role of PsrA in P. aeruginosa PAO1.

2024-07-05 | GSE249518 | GEO

Pseudomonas aeruginosa PAO1 response to sphingomyelin

Project description:Analysis of Pseudomonas aeruginosa PAO1 treated with 200 µM sphingomyelin. Results provide insight into the response to sphingomyelin in P. aeruginosa.

2017-02-12 | GSE83078 | GEO

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