Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Characterization of glyceollins as novel aryl hydrocarbon receptor ligands and their role in cell migration

ABSTRACT: Characterization of glyceollins as novel aryl hydrocarbon receptor ligands and their role in cell migration

PROVIDER: PRJNA594408 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10614600.fastq.gz	Fastqsanger.gz
	SRR10614601.fastq.gz	Fastqsanger.gz
	SRR10614602.fastq.gz	Fastqsanger.gz
	SRR10614603.fastq.gz	Fastqsanger.gz
	SRR10614604.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 90

Similar Datasets

Zebrafish larvae aryl hydrocarbon receptor ligands and inhibitor

Project description:Zebrafish larvae aryl hydrocarbon receptor ligands and inhibitor

| PRJNA495640 | ENA

Zebrafish larvae aryl hydrocarbon receptor ligands and inhibitor

Project description:Wild type zebrafish (AB strain, https://zfin.org/action/genotype/view/ZDB-GENO-960809-7) were set up for mating overnight, in 3:2 ratios, females and males, respectively. Fertilized embryos from wildtype zebrafish AB strain were used for all experiments and kept in Embryo medium (E3, 5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl2, 0.33 mM MgSO4) in an incubator at 28ºC. At 1dpf, larvae were manually dechorionated under a stereomicroscope. At 2dpf, zebrafish larvae were divided randomly into the different experimental groups, at a density of 12 larvae per well of a 12 well plate (Corning) in 1.5mL of E3. Three biological replicates of 12 larvae per condition were exposed to different conditions for 4 hours at 28 ºC: DMSO; DMSO + CH223191 (5mM); 3-o-C12-L-HSL (50mM); 3-o-C12-L-HSL (50mM) + CH223191 (5 mM ); 1-hydroxyphenazine (1-HP, 5mM); 1-HP (5mM) + CH223191(5mM) and 1-HP (5mM) + 3-o-C12-L-HSL (50mM).To mention, in experiments using the Aryl hydrocarbon Receptor inhibitor CH223191, larvae were pre-exposed to 5uM of the inhibitor for 2h prior to the start of the experiment and the inhibitor was kept during the experiment. After the desired exposure to diverse ligands, larvae were euthanized with Tricaine (MS-222, 300 µg/mL) and placed in Trizol for RNA isolation. The same experimental layout was performed 5 times, and samples were subjected to microarray hybridization and analysis.

2019-10-01 | GSE121101 | GEO

Aryl Hydrocarbon Receptor Ligands Present In The Environment Impact Human Trophoblast Cell Development

Project description:Aryl Hydrocarbon Receptor Ligands Present In The Environment Impact Human Trophoblast Cell Development

| PRJNA1033546 | ENA

Lead optimization of aryl hydrocarbon receptor ligands for treatment of inflammatory skin disorders

Project description:Lead optimization of aryl hydrocarbon receptor ligands for treatment of inflammatory skin disorders

| PRJNA875926 | ENA

Ancestralization of the modern human aryl hydrocarbon receptor by genome editing

Project description:The aryl hydrocarbon receptor (AHR) is a transcription factor that upon binding ligands induces the expression of cytochrome P450 genes, which encode enzymes that metabolize aromatic hydrocarbons. The AHR carries an amino acid substitution at position 381 in all present-day humans that occurred after the divergence of modern humans from Neandertals and Denisovans. Here, we use genome editing to revert position 381 back to the ancestral state in human cells. Cells were treated with ligands for 4 h followed by RNA extraction and RNA-Seq.

2024-06-26 | GSE210514 | GEO

Rutaecarpine inhibits glioblastoma migration by activating the aryl hydrocarbon receptor (AhR) signaling pathway

Project description:Glioblastoma is the most frequent and aggressive primary astrocytoma in adults. The high migration ability of the tumor cells is an important reason for the high recurrence rate and poor prognosis of glioblastoma. Recently, emerging evidence has shown that the migration ability of glioblastoma cell was inhibited upon the activation of aryl hydrocarbon receptor (AhR), suggesting potential anti-tumor effects of AhR agonists. Rutaecarpine is a natural compound with potential tumor therapeutic effects which can possibly bind to AhR. However, its effect on the migration of glioblastoma is unclear. Therefore, we aim to explore the effects of rutaecarpine on the migration of human glioblastoma cells U87 and the involvements of the AhR signaling pathway. The results showed that: (i) compared with other structural related alkaloids, like evodiamine and dehydroevodiamine, rutaecarpine was a more potent AhR activator, and has stronger inhibitory effect on the glioblastoma cell migration; (ii) rutaecarpine decreased the migration ability of U87 cells in an AhR-dependent manner; (iii) AhR mediated the expression of a tumor suppressor interleukin 24 (IL24) induced by rutaecarpine, and AhR-IL24 axis was involved in the anti-migratoryeffects of rutaecarpine on the glioblastoma. Besides IL24, other motility related genes were proposed to participate in the migration regulation process of rutaecarpine by RNA-Seq analysis. These data suggest that rutaecarpine is a natural AhR agonist that could inhibit the migration of glioblastomaandbe a potential candidate for developing therapeutic drug against glioblatoma.

2021-12-07 | GSE183606 | GEO

The Aryl Hydrocarbon Receptor Regulates Tissue-Specific Dioxin-Dependent and Dioxin-Independent Gene Batteries

Project description:This SuperSeries is composed of the following subset Series: GSE15857: The Aryl Hydrocarbon Receptor Regulates Tissue-Specific Dioxin-Dependent and Dioxin-Independent Gene Batteries: Kidney GSE15858: The Aryl Hydrocarbon Receptor Regulates Tissue-Specific Dioxin-Dependent and Dioxin-Independent Gene Batteries: Liver Refer to individual Series

2010-05-05 | E-GEOD-15859 | biostudies-arrayexpress

Aryl Hydrocarbon Receptor Ligands Present In The Environment Impact Human Trophoblast Cell Development [STEM]

Project description:Aryl Hydrocarbon Receptor Ligands Present In The Environment Impact Human Trophoblast Cell Development [STEM]

| PRJNA1033553 | ENA

Aryl Hydrocarbon Receptor Ligands Present In The Environment Impact Human Trophoblast Cell Development [EVT]

Project description:Aryl Hydrocarbon Receptor Ligands Present In The Environment Impact Human Trophoblast Cell Development [EVT]

| PRJNA1033543 | ENA

Aryl Hydrocarbon Receptor Ligands Present In The Environment Impact Human Trophoblast Cell Development [ST3D]

Project description:Aryl Hydrocarbon Receptor Ligands Present In The Environment Impact Human Trophoblast Cell Development [ST3D]

| PRJNA1033542 | ENA