Project description:The expression levels of developing mouse molar transcriptome were measured. 5 samples of both E13.5 and E14.5 of mouse developing molar tooth.
Project description:The expression levels of developing rat molar transcriptome were measured. 5 samples each from embryonic day 15.5, and 17.5 of rat developing molar tooth.
Project description:The expression levels of developing mouse molar transcriptome were measured. 7 samples each from embryonic day 13.5, 14.5 and 16.5 of mouse developing molar tooth.
Project description:To identify genes heretofore undiscovered as critical players in the biogenesis of teeth, we have used microarray gene expression analysis of the developing mouse molar tooth (DMT) between 1 and 10 days postnatal to identify genes differentially expressed when compared to 16 control tissues (GEO accession # GSE1986). Of the top 100 genes exhibiting increased expression in the DMT, 29 were found to have been previously associated with tooth development. Differential expression of the remaining 71 genes not previously associated with tooth development was confirmed by qRT-PCR analysis. Further analysis of seven of the latter genes by mRNA in situ hybridization found that five were specific to the developing tooth in the craniofacial region (Rspo4, Papln, Amtn, Gja1, Maf). Of the remaining two, one was found to be more widely expressed (Sp7) and the other was found to be specific to the nasal serous gland, which is close to, but distinct from, the developing tooth (Vrm). Keywords: tooth development