Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic profiling of murine Nfe2l2 knockout melanoma cells

ABSTRACT: Transcriptomic profiling of murine Nfe2l2 knockout melanoma cells

PROVIDER: PRJNA595165 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10989400.fastq.gz	Fastqsanger.gz
	SRR10989401.fastq.gz	Fastqsanger.gz
	SRR10989402.fastq.gz	Fastqsanger.gz
	SRR10989403.fastq.gz	Fastqsanger.gz
	SRR10989404.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Transcriptomic profiling of murine Nfe2l2 knockout melanoma cells

Project description:The role of Nfe2l2 in established murine melanoma cells was studied by analyzing expression profiles of control and Nfe2l2 knockout melanoma cells, generated by CRISPR/Cas9.

2020-10-01 | GSE141912 | GEO

Patient-Derived Mutant Forms of NFE2L2/NRF2 Drive Aggesssive Murine Hepatoblastomas

Project description:The pediatric liver cancer hepatoblastoma (HB) often expresses mutant forms of b-catenin and dysregulates the Hippo tumor suppressor pathway. Murine HBs can be generated by co-expressing b-catenin mutants and the constitutively active Hippo effector YAPS127A. Some human HBs also express mutant forms of NFE2L2/NRF2 (NFE2L2), a bHLH transcription factor that tempers oxidative and electrophilic stress. In doing so, NFE2L2 either suppresses or facilitates tumorigenesis in a context- and time-dependent manner. We show here that two patient-derived NFE2L2 missense mutants, L30P and R34P, markedly accelerate HB growth in association with diffuse cyst formation, an otherwise uncommon feature of human HB. Surprisingly, we found that any two members of the mutant b-catenin-YAPS127A-L30P/R34P triad were tumorigenic, thus providing direct evidence for NFE2L2’s oncogenicity. Each tumor group showed distinct features but shared a similarly deregulated set of 22 transcripts, 10 of which perfectly correlated with survival in human HBs. 17 transcripts also correlated with survival in multiple cancers. One of the most deregulated transcripts encoded Serpin E1, a serine protease inhibitor with roles in fibrinolysis, tumor growth and extracellular matrix formation. The combination of mutant b-catenin-YAPS127A-Serpin E1 neither accelerated tumor growth nor generated cysts but did promote large-scale necrosis that was often associated with the cysts seen in mutant b-catenin-YAPS127A-L30P/R34P tumor, albeit on a lesser scale. These findings establish the direct oncogencity of NFE2L2 mutants and identify key transcripts, including Serpin E1, that impact tumor growth and other HB features.

2021-05-20 | GSE157623 | GEO

Patient-Derived Mutant Forms of NFE2L2/NRF2 Drive Aggesssive Murine Hepatoblastomas

Project description:Patient-Derived Mutant Forms of NFE2L2/NRF2 Drive Aggesssive Murine Hepatoblastomas

| PRJNA662245 | ENA

Expression profile of TRAMP-C1 cell line with PAX8-NFE2L2 overexpression

Project description:We synthesized the PAX8-NFE2L2 fusion transcript and cloned it into a lentiviral vector, and used this to overexpress it in the murine prostate adenocarcinoma cell line TRAMP-C1.

2016-11-24 | GSE79756 | GEO

DNMT1 KNOCKOUT IN MURINE LIVER

Project description:DNMT1 KNOCKOUT IN MURINE LIVER

| PRJNA280860 | ENA

Molecular Analysis of Murine KitK641E Melanoma Progression

Project description:Molecular Analysis of Murine KitK641E Melanoma Progression

| PRJNA1067722 | ENA

Proteomics of murine melanoma tumor cells upon sphingosine kinase-1 inhibition

Project description:In order to assess the impact of sphingosine kinase-1 (SK1) expression on melanoma growth, stable SK1 knockdown cells were generated by shRNA, using Yumm 1.7 cells derived from spontaneous murine melanoma driven by Braf activation as well as Pten and Cdkn2a inactivation. Two cell lines silenced for SK1 (shSK1(1) and shSK1(2)) were obtained.

2019-12-17 | PXD016535 | Pride

Gene expression profiling of murine tumors derived from Renca control knockout vs Pbrm1 knockout tumor clones

Project description:We investigated the influence of Pbrm1 on the tumor microenvironment in murine tumors. We employed a Renca-BALB/c murine immune competent model for our study. We compared Renca control knockout tumors to two Pbrm1 KO tumor clones (n=5 for each of two clones).

2020-02-26 | GSE145919 | GEO

Analysis of NFE2L2 (NRF2) binding to chromatin in primary human keratinocytes

Project description:Analysis of NFE2L2 (NRF2) binding to chromatin in primary human keratinocytes

2021-03-31 | GSE150760 | GEO

Transcriptome profiling of murine aortic Vascular Smooth Muscle cells from ApoE knockout and ApoE/Neil3 double knockout mice

Project description:We performed high-throughput RNA sequencing to characterize possible differences in the transcriptome of murine aortic Vascular Smooth Muscle cells from both 26 weeks-old ApoE knockout and ApoE/Neil3 double knockout mice fed a regular diet.

2021-03-15 | GSE140790 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data