Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of human and mouse brain microvessels isolated by laser capture microdissection and matched whole brain samples

ABSTRACT: RNA-seq of human and mouse brain microvessels isolated by laser capture microdissection and matched whole brain samples

PROVIDER: PRJNA596224 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10721155.fastq.gz	Fastqsanger.gz
	SRR10721156.fastq.gz	Fastqsanger.gz
	SRR10721157.fastq.gz	Fastqsanger.gz
	SRR10721158.fastq.gz	Fastqsanger.gz
	SRR10721159.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

RNA-seq of human and mouse brain microvessels isolated by laser capture microdissection and matched whole brain samples

Project description:Brain microvessels form the blood-brain barrier, and are dysfunctional in several neurological disorders. Brain microvessels are formed by brain microvascular endothelial cells (BMECs) and pericytes, and the molecular constituents of these cell types remain incompletely characterized, especially in humans. To improve molecular knowledge of these cell types and identify species differences in gene expression, we performed RNA-sequencing on brain microvessels isolated from human and mouse tissue samples using laser capture microdissection. We also performed RNA-sequencing of matched whole brain samples to identify genes with microvessel-enriched expression.

2020-07-23 | GSE142209 | GEO

Microarray analysis of rat hippocampal microvessels at acute time points following focused ultrasound-induced increase in blood-brain barrier permeability

Project description:Therapeutic treatment options for central nervous system diseases are greatly limited by the blood-brain barrier (BBB). Focused ultrasound (FUS), in conjunction with circulating microbubbles, can be used to induce a targeted and transient increase in BBB permeability, providing a unique approach for the delivery of drugs from the systemic circulation into the brain. While preclinical research has demonstrated the utility of FUS, there remains a large gap in our knowledge regarding the impact of sonication on BBB gene expression. This work is focused on investigating the transcriptional changes in dorsal hippocampal rat microvessels in the acute stages following sonication. Microarray analysis of microvessels was performed at 6 and 24 hrs post-FUS. Microvessels were collected by laser capture microdissection. Relative gene expression was compared between samples collected from the sonicated hemisphere and samples collected from the hemisphere contralateral to sonication, as well as samples collected from rats not undergoing the FUS procedure.

2017-09-01 | E-MTAB-5251 | biostudies-arrayexpress

North American Brain Expression Consortium: Laser capture microdissection of Cerebellar Purkinje Cells

Project description:Previous studies in bulk tissue suggest that there are abundant expression quantitative trait loci (eQTLs) in human brain. This sample series is of cerebellar Purkinje cells isolated using laser capture microdissection from human cases without neurological disease but of known genotypes. These data may be helpful in confirming eQTLs in bulk tissue or in mapping other gene expression traits in an enriched neuronal population. Authorized Access data: Mapping of GEO sample accessions to dbGaP subject/sample IDs is available through dbGaP Authorized Access, see http://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs000249 The aim of this study was to examine gene expression in isolated purkinje cells from the human cerebellum. We obtained frozen brain tissue from the cerebellum. We stained sections with cresyl violet and separated Purkinje cells based on morphology and location within the cerebellum using laser capture microdissection. Expression analyses were then performed.

2013-01-01 | E-GEOD-37205 | biostudies-arrayexpress

Defining the Phosphodiesterase Superfamily Members in Rat Brain Microvessels

Project description:A filtration method followed by microarray analyses allows PDE components to be identified in brain microvessels, and confirmed that PDE4D and PDE5A are the primary forms expressed in rat brain microvessels. Adult male F344 rats were sacrificed and blocks of the cerebral cortex and infratentorial areas were dissected. Microvessels were isolated using a filtration method, and total RNA was extracted. Two microarrays using four sample RNAs (microvessels from the cerebral cortex vs. microvessels from the infratentorial block) were included in the present study. Microarrays demonstrated that there were 16 PDE transcripts in the PDE superfamily, exhibiting quantifiable density in the microvessels.

2012-02-01 | E-GEOD-35285 | biostudies-arrayexpress

North American Brain Expression Consortium: Laser capture microdissection of Cerebellar Purkinje Cells

2013-01-01 | GSE37205 | GEO

Laser capture microdissection of endothelial and neuronal cells from human dorsolateral prefrontal cortex

Project description:We used laser capture microdissection to isolate both microvascular endothelial cells and neurons from post mortem brain tissue from patients with schizophrenia and bipolar disorder and healthy controls. RNA was isolated from these cell populations, amplified, and analysed using Affymetrix HG133plus2.0 GeneChips. In the first instance, we used the dataset to compare the neuronal and endothelial data, in order to demonstrate that the predicted differences between cell types could be detected using this methodology. Keywords: cell type comparison, laser capture microdissection

2008-12-18 | GSE12679 | GEO

Gene expression profiling of the retinal microvessels and the brain microvessels

Project description:Rat retinal microvessels (RMVs) and brain microvessels (BMVs) were mechanically isolated under ice-cold condition. Retinal and brain tissues (RT, BT) from the same animals were collected for comparisons. Total RNA were individually extracted, amplified and processed on Affymetrix rat 2.0 microarray Chips. We sought to obtain the whole gene expression profile of RMVs, RT, BMVs and BT. Differently expressed genes (DEGs) between RMVs and RT, and between RMVs and BMVs, between RT and BT were analyses. Using these DEGs, we comprehensively analyzed gene expression of the microvessels and highlighted their involvement in critical functional structures in RMVs and BMVs, such as junctional complex, transporters and signaling pathways.

2019-10-16 | GSE110675 | GEO

Defining the Phosphodiesterase Superfamily Members in Rat Brain Microvessels

2012-02-01 | GSE35285 | GEO

Gene expression profiling of the retinal microvessels and the brain microvessels

Project description:Gene expression profiling of the retinal microvessels and the brain microvessels

| PRJNA434237 | ENA

Brain microvessels ontogeny-ERI-INSERM28

Project description:Enriched forebrain microvessels from P5, P10 and adult mice. Transcript comparisons identified 5873 genes with FC>2 between ages and 93 pathways. The observations were confronted to proteome study using NanoLS-MS/MS and deposited on proteomeXchange platform under # PXD001718. The 2 techniques converge towards extracellular matrix, cell adhesion and junction pathways, indicating early changes in development. The difference are examined as putative structural basis of very preterm intracerbral hemorrhage, 3 age comparisons of 5 days, 10 days and adult brain microvessels, 2 replicates, Agilent Whole Mouse Genome Oligo 4Ã44K Microarray

2016-01-10 | E-GEOD-67870 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data