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ABSTRACT: Biogeographic Barriers, Pleistocene Refugia, and Climatic Gradients in the Southeastern Nearctic Drive Diversification in Cornsnakes (Pantherophis guttatus complex)

PROVIDER: PRJNA596278 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10724500.fastq.gz	Fastqsanger.gz
	SRR10724501.fastq.gz	Fastqsanger.gz
	SRR10724502.fastq.gz	Fastqsanger.gz
	SRR10724503.fastq.gz	Fastqsanger.gz
	SRR10724504.fastq.gz	Fastqsanger.gz

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Metabolomic evidence for biogeographic segregation in Espeletiinae (Asteraceae): A new perspective on an Andean adaptive radiation in sky islands (Intraspecific chemical variability assay)

Project description:The biogeography of tropical Andean plants and the effects of altitudinal barriers as evolutionary constraints have been the focus of great debate. Although genetic markers have provided evidence for the geographic isolation of Andean-endemic groups, even highly variable molecular markers commonly used to assess biogeography do not have the resolving power needed for a fine geographic discrimination in some Andean taxa. We present a metabolomics approach based on ultrahigh-performance liquid chromatography-mass spectrometry combined with multivariate statistical methods and machine learning algorithms of multiple species and populations of Espeletiinae (Asteraceae) to provide metabolomic evidence for biogeographic segregation in this Andean-endemic subtribe. Our approach allows the discrimination of Espeletiinae taxa at different geographic scales with characteristic metabolic fingerprints related to their country of origin on a global scale, to their páramo massifs on a regional scale and to their páramo complexes on a local scale, revealing inter- and intraspecific metabolic variations. Our results, together with previous studies, suggest that Andean geography and Pleistocene glacial cycles not only shaped the evolutionary history of Espeletiinae but also its metabolic fingerprints, demonstrating the potential of metabolomics for understanding biogeographic patterns in recently diversified plant groups where genetic divergence is still at an early stage. Intraspecific chemical variability assay protocols and data are reported in the current study <a href='https://www.ebi.ac.uk/metabolights/MTBLS943' rel='noopener noreferrer' target='_blank'>MTBLS943</a>.Interspecific chemical variability assay protocols and data are reported in study <a href='https://www.ebi.ac.uk/metabolights/MTBLS944' rel='noopener noreferrer' target='_blank'>MTBLS944</a>.

2020-03-24 | MTBLS943 | MetaboLights

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