Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Construction of PYCARD-inducible system in two cancer cell lines, LNCaP (prostate cancer) and DLD1 (colorectal cancer)

ABSTRACT: Construction of PYCARD-inducible system in two cancer cell lines, LNCaP (prostate cancer) and DLD1 (colorectal cancer)

PROVIDER: PRJNA596468 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Construction of PYCARD-inducible system in two cancer cell lines, LNCaP (prostate cancer) and DLD1 (colorectal cancer)

Project description:Epigenetic gene silencing by aberrant DNA methylation is one of the important mechanisms leading to the loss of key cellular pathways in tumorigenesis. We previously reported that PYCARD (PYD and CARD domain containing), an apoptosis inducing gene, was frequently hypermethylated in prostate cancer in a tumor-specific manner (46/51: 90%), by methylation specific PCR (MSP). In order to examine the relationship between methylation status and level of the PYCARD protein expression in prostate cancer, we further performed immunohistochemical analyses of the 51 MSP-analyzed prostate cancer specimens and observed reduced or absent protein expression in tumors with aberrant methylation status of PYCARD. In contrast, PYCARD was unmethylated and expressed in normal prostatic tissues including epithelial, basal and inflammatory cells. Furthermore, we tried to identify downstream genes of PYCARD by microarray analyses using PYCARD inducible LNCaP prostate cancer cells. Interestingly, PYCARD induction upregulated PYDC1, another PYCARD homolog that associates with PYCARD and accelerates inflammation and apoptosis. Our present results suggest that aberrant methylation of PYCARD is an extremely frequent event and contributes to escape from apoptosis by reducing PYCARD expression in prostate cancer.

2020-12-19 | GSE142283 | GEO

FOXO3a and beta-catenin transcriptional profile in human colon cancer cell line DLD1

Project description:We established a model system in human DLD1 colon cancer cells to study the transcriptional crosstalk between FOXO3A and beta-Catenin. Thereby, translocation to the nucleus of a AKT-insensitive mutant (T32A, S253A, S315A) of human FOXO3A fused to the ligand binding domain of human estrogen receptor can be induced by exposure to 4-hydroxy-tamoxifen. Furthermore, expression of a stable mutant (S33Y) of human beta-catenin is doxycycline inducible. Addition of those drugs separately or in combination allows identification of common or excusive target gene sets.

2012-05-01 | E-MEXP-3262 | biostudies-arrayexpress

LNCaP prostate cancer cells

Project description:LNCaP prostate cancer cells

| PRJNA801659 | ENA

Differential Gene expression of furin knockout (KO) DLD1, HCA7 and HT29 colorectal cancer cells

Project description:We created furin KO cells in three colorectal cancer cell lines (DLD1, HCA7 and HT29) using CRISPR-Cas9 genome editing. And then we performed RNA-Seq analysis in furin KO colorectal cancer cells to identify potential effect of furin on gene expression patterns in DLD1, HCA7 and HT29 cells.

2020-03-15 | GSE130969 | GEO

DLD1

Project description:DLD1 is an APC mut, KRAS mut, P53 mut CRC cell line. PROX1 transcription factor, target of Wnt pathway in CRC, is our protein of interest.DLD1 cells are PROX1 negative. We overexpressed through lentiviral expression PROX1 protein or the empty vector psd44, through selection of the cells in puromycin resistance. Afterwards we compared the transcriptional program of the DLD1-PROX1 and DLD1-Control cells growing in monolayer in vitro.

2014-09-18 | E-MTAB-2020 | biostudies-arrayexpress

Gene expression induced by 5SA-Atoh1 in DLD1 cells

Project description:To investigate the function of Atoh1 in colon cancer cells. We have employed whole genome microarray expression profiling as a discovery platform to iedntify the gene expression induced by Atoh1 in colon cancer cells. Mutated Atoh1 (5SA-Atoh1) replaced 5 serine residues to Alanin for the protein stabilization were expressed in human colon cancer cellline; DLD1 cells. Triplicated RNAs were generated from GFP expressing DLD1 cells and 5SA-Atoh1 expressing DLD1 cell, respectively.

2012-04-10 | E-GEOD-37143 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of DLD1 parental cells and DLD1 p85β K477A/R478A mutant cells [RNA-seq]

Project description:To elucidate whether p85β nuclear translocation plays a role in cancer tumorigenesis, a RNA-seq analysis was performed to compare the gene expression profiles of DLD1 parental cells and DLD1 p85β K477A/R478A mutant cells

2021-12-15 | GSE190740 | GEO

Construction of IRX4-inducible system in pancreatic cancer cell lines, PK-1 and PK-9

Project description:Construction of IRX4-inducible system in pancreatic cancer cell lines, PK-1 and PK-9

| PRJNA592956 | ENA

Expression data from YAP-S127D DLD1 Xenografts

Project description:We overexpressed YAP-S127D in DLD1 colorectal cancer cells for 21 days after sub-cutaneous (SubQ) injection into the flanks of nude mice.

2012-11-29 | GSE41508 | GEO

Expression data form PI3K Knockout colon cancer cell lines (HCT116 and DLD1)

Project description:Whole transciptome analysis of colon cancer mutated cell lines(HCT116 and DLD1) under serum starvation conditions (19hrs-0.5%FBS) We used microarrays to compare gene regulation of truncated cell lines, knocked-out of either the wild type or mutated allele of PI3K, for two independent colon cancer cell lines

2022-12-31 | GSE58904 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data