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Alteration in the Culex pipiens transcriptome reveals the implication of the mosquito immune system during Rift Valley fever phlebovirus infection

ABSTRACT: Alteration in the Culex pipiens transcriptome reveals the implication of the mosquito immune system during Rift Valley fever phlebovirus infection

PROVIDER: PRJNA596664 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10741104_1.fastq.gz	Fastqsanger.gz
	SRR10741104_2.fastq.gz	Fastqsanger.gz
	SRR10741105_1.fastq.gz	Fastqsanger.gz
	SRR10741105_2.fastq.gz	Fastqsanger.gz
	SRR10741106_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Alteration in the Culex pipiens transcriptome reveals the implication of the mosquito immune system during Rift Valley fever phlebovirus infection

Project description:Rift valley fever (RVF) is an emerging zoonotic disease and it is caused by Rift valley fever phlebovirus (RVFV). This virus is commonly transmitted in endemic areas between wild ruminants and mosquitoes, mainly by mosquitoes of Culex and Aedes genus. Starting from 2000, several outbreaks have been reported outside the African continent, in countries facing the Mediterranean Sea, such as Saudi Arabia. The available vaccines for ruminants present limited efficacy or residual pathogenic effects. Consequently, new strategies are urgently required to limit the expansion of this zoonotic virus. The main objective of this work is to investigate the molecular responses of Culex pipiens to RVFV focusing mainly on genes implicated in the classical innate immunity pathways, RNAi mechanism and apoptosis process in order to elucidate the implicated genes in viral infection. The immune altered genes here described could be potential targets to control RVFV infection in mosquitoes. Some of the genes related to the immune defense response were previously described in others mosquito-arbovirus models, as also in Drosophila and human. To our knowledge, this study elucidates for the first time the Cx. pipiens-RVFV interaction in terms of defense infection-response, which was largely under studied and provides information to develop new approaches to prevent and control the expansion of the virus in the future.

2020-11-02 | GSE142339 | GEO

Culex pipiens transcriptome assembly

Project description:Alteration in the Culex pipiens transcriptome reveals the implication of the mosquito immune system during Rift Valley fever phlebovirus infection

| PRJEB35657 | ENA

RNA-sequencing of Culex pipiens molestus and Culex pipiens quinquefasciatus

Project description:Culex pipiens molestus and Cx. p. quinquefasciatus are the members of Culex pipiens Complex, but they display relatively large differences in behavior and physiological responses. We compared the genes of these mosquitoes to identify those that were differentially expressed in each subspecies. Such genes could play important roles in subspecies-specific blood feeding or oviposition behavior.

2014-06-26 | GSE58835 | GEO

RNA-sequencing of Culex pipiens molestus and Culex pipiens quinquefasciatus

2014-06-26 | E-GEOD-58835 | biostudies-arrayexpress

Culex flavivirus infection in a Culex pipiens mosquito colony and its effects on vector competence for Rift Valley fever phlebovirus.

Project description:Rift Valley fever is a mosquito-borne zoonotic disease that affects domestic ruminants and humans. Culex flavivirus is an insect-specific flavivirus that naturally exists in field mosquito populations. The influence of Culex flavivirus on Rift Valley fever phlebovirus (RVFV) vector competence of Culex pipiens has not been investigated.Culex flavivirus infection in a Cx. pipiens colony was studied by Culex flavivirus oral feeding and intrathoracical inoculation. Similarly, vector competence of Cx. pipiens infected with Culex flavivirus was evaluated for RVFV. Infection, dissemination, transmission rates and transmission efficiency of Culex flavivirus-infected and non-infected Cx. pipiens artificially fed with RVFV infected blood were assessed.Culex flavivirus was able to infect Cx. pipiens after intrathoracically inoculation in Cx. pipiens mosquitos but not after Culex flavivirus oral feeding. Culex flavivirus did not affect RVFV infection, dissemination and transmission in Cx. pipiens mosquitoes. RVFV could be detected from saliva of both the Culex flavivirus-positive and negative Cx. pipiens females without significant differences. Moreover, RVFV did not interfere with the Culex flavivirus infection in Cx. pipiens mosquitoes.Culex flavivirus infected and non-infected Cx. pipiens transmit RVFV. Culex flavivirus existing in field-collected Cx. pipiens populations does not affect their vector competence for RVFV. Culex flavivirus may not be an efficient tool for RVFV control in mosquitoes.

| S-EPMC5966921 | biostudies-literature

Culex pipiens pipiens

Project description:Culex pipiens pipiens assembly and annotation

| PRJNA1074129 | ENA

Culex pipiens pipiens

Project description:Culex pipiens pipiens Transcriptome or Gene expression

| PRJNA275017 | ENA

Culex pipiens

Project description:Culex pipiens overview

| PRJNA266849 | ENA

Characterization of nucleocapsid-RNA interactions in Rift Valley fever virus infected cells

Project description:Crosslinking immunoprecipitation and sequencing was used to characterize nucleocapsid-RNA interactions in Rift Valley fever virus infection. This data set includes illumina HiSeq paired-end reads of Rift Valley fever virus infected HEK293 cells. The sequencing libraries were generated from nucleocapsid-bound RNAs.

2021-10-22 | GSE186067 | GEO

Alteration in the Culex pipiens transcriptome reveals diverse mechanisms of the mosquito immune system implicated upon Rift Valley fever phlebovirus exposure.

Project description:Rift Valley fever phlebovirus (RVFV) causes an emerging zoonotic disease and is mainly transmitted by Culex and Aedes mosquitoes. While Aedes aegypti-dengue virus (DENV) is the most studied model, less is known about the genes involved in infection-responses in other mosquito-arboviruses pairing. The main objective was to investigate the molecular responses of Cx. pipiens to RVFV exposure focusing mainly on genes implicated in innate immune responses. Mosquitoes were fed with blood spiked with RVFV. The fully-engorged females were pooled at 3 different time points: 2 hours post-exposure (hpe), 3- and 14-days post-exposure (dpe). Pools of mosquitoes fed with non-infected blood were also collected for comparisons. Total RNA from each mosquito pool was subjected to RNA-seq analysis and a de novo transcriptome was constructed. A total of 451 differentially expressed genes (DEG) were identified. Most of the transcriptomic alterations were found at an early infection stage after RVFV exposure. Forty-eight DEG related to immune infection-response were characterized. Most of them were related with the RNAi system, Toll and IMD pathways, ubiquitination pathway and apoptosis. Our findings provide for the first time a comprehensive view on Cx. pipiens-RVFV interactions at the molecular level. The early depletion of RNAi pathway genes at the onset of the RVFV infection would allow viral replication in mosquitoes. While genes from the Toll and IMD immune pathways were altered in response to RVFV none of the DEG were related to the JAK/STAT pathway. The fact that most of the DEG involved in the Ubiquitin-proteasome pathway (UPP) or apoptosis were found at an early stage of infection would suggest that apoptosis plays a regulatory role in infected Cx. pipiens midguts. This study provides a number of target genes that could be used to identify new molecular targets for vector control.

| S-EPMC7755283 | biostudies-literature

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