Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: RNASeq of Cyclopterus lumpus (lumpfish)

PROVIDER: PRJNA596743 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10740658_1.fastq.gz	Fastqsanger.gz
	SRR10740658_2.fastq.gz	Fastqsanger.gz
	SRR10740659_1.fastq.gz	Fastqsanger.gz
	SRR10740659_2.fastq.gz	Fastqsanger.gz
	SRR10740660_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 54

Similar Datasets

fCycLum

Project description:Cyclopterus lumpus (lumpfish)

| PRJEB65376 | ENA

Lumpfish (Cyclopterus lumpus) genome assembly

Project description:Lumpfish (Cyclopterus lumpus) genome assembly

| PRJEB29001 | ENA

Project description:Cyclopterus lumpus (Lumpfish) genome, fCycLum1

| PRJNA562201 | ENA

fCycLum2

Project description:Cyclopterus lumpus (lumpfish) genome assembly, fCycLum2

| PRJEB66140 | ENA

Cyclopterus lumpus isolate:fCycLum1

Project description:Cyclopterus lumpus (Lumpfish) genoem, fCycLum1, alternate haplotype

| PRJNA562004 | ENA

fCycLum

Project description:Cyclopterus lumpus (lumpfish), genomic and transcriptomic data

| PRJEB65375 | ENA

Cyclopterus lumpus isolate:fCycLum1

Project description:Cyclopterus lumpus (Lumpfish) genome, fCycLum1, primary haplotype

| PRJNA562003 | ENA

Transcriptome-wide analyses of early immune responses in lumpfish leukocytes upon stimulation with poly(I:C)

Project description:Both bacterial and viral diseases are a major threat to farmed fish, as well as wild fish. As the antiviral immune mechanisms in lumpfish (Cyclopterus lumpus L.) are poorly understood, lumpfish leukocytes were stimulated with poly(I:C), a synthetic analog of virus dsRNA, and RNA sequencing was performed. Immune genes were identified, and transcriptome-wide analyses of early immune responses in lumpfish leukocytes showed that 310 and 1872 transcripts were significantly differentially expressed 6 and 24 hours post exposure (hpe) to poly(I:C), respectively. The most enriched GO terms when time had been accounted for, were immune system processes (GO:0002376) and immune response (GO:0006955). Analysis of differentially expressed genes (DEGs) showed that among the most highly upregulated genes were TLRs and genes belonging to the RIG-I signaling pathway, including LGP2, MDA5, TRIM25, STING, as well as IRF3, IL-8 and TNFα. RIG-I was not identified, but in silico analyses showed that genes encoding proteins involved in pathogen recognition, cell signaling, and cytokines of the TLR and RIG-I signaling pathway are mostly conserved in lumpfish when compared to mammals and other teleost species. Our analyses unravel the innate immune pathways playing a major role in antiviral defence in lumpfish. The information gathered can be used to compare research findings and lay the groundwork for future functional analyses of immune and pathogenicity mechanisms. Such knowledge is also necessary for the development of immunoprophylactic measures for lumpfish, which is extensively cultivated for use as cleaner fish in the aquaculture for removal of sea lice from Atlantic salmon (Salmo salar).

2023-06-01 | E-MTAB-12884 | biostudies-arrayexpress

fCycLum2 alternate haplotype

Project description:Cyclopterus lumpus (lumpfish) genome assembly, fCycLum2, alternate haplotype

| PRJEB66139 | ENA

Cyclopterus lumpus

Project description:Cyclopterus lumpus overview

| PRJNA562361 | ENA