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DM-tRNA-seq of mock and MHV68-infected mouse MC57G fibroblasts

ABSTRACT: DM-tRNA-seq of mock and MHV68-infected mouse MC57G fibroblasts

PROVIDER: PRJNA596806 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10742263_1.fastq.gz	Fastqsanger.gz
	SRR10742263_2.fastq.gz	Fastqsanger.gz
	SRR10742264_1.fastq.gz	Fastqsanger.gz
	SRR10742264_2.fastq.gz	Fastqsanger.gz
	SRR10742265_1.fastq.gz	Fastqsanger.gz

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DM-tRNA-seq of mock and MHV68-infected mouse MC57G fibroblasts

Project description:We applied a tRNA-specific sequencing pipeline to analyze tRNA expression during infection of MC57G fibroblasts with the gammaherpesvirus MHV68. We find that expression of 14% of tRNA genes is significantly upregulated during infection. By mapping reads to either premature tRNA (pre-tRNA) or mature tRNA sequences, we find that increased expression of tRNA genes is manifested at the level of the pre-tRNA only, indicating a homeostatic mechanism to maintain the size and composition of mature tRNA pools.

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