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Expression data from ex vivo and cultured adipose-derived mesenchymal stromal cells (AdMSC, also known as ASC)

ABSTRACT: Expression data from ex vivo and cultured adipose-derived mesenchymal stromal cells (AdMSC, also known as ASC)

PROVIDER: PRJNA597007 | ENA |

REPOSITORIES: ENA

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Expression data from ex vivo and cultured adipose-derived mesenchymal stromal cells (AdMSC, also known as ASC)

Project description:The effect of standard tissue culture on AdMSC is not well defined, therefore we sought to analyse global changes in microRNA expression when a defined ex vivo population of AdMSC was subjected to standard tissue culture conditions.

2019-12-21 | GSE142429 | GEO

Adipose-derived stromal/stem cells (ASC)

Project description:Adipose-derived stromal/stem cells (ASC) capable of multipotential differentiation can be isolated with high yield from human subcutaneous lipoaspirates. This study reports our recent experience isolating and immunophenotypically characterizing ASCs from >60 human subjects

2010-10-01 | GSE18391 | GEO

Gene expression profiles of human retinal microvascular pericytes (HRMVPC) and human lipoaspirate derived mesenchymal stromal cells (adipose stromal cells, ASC)

Project description:Comparative gene expression profiles of human retinal pericytes (HRMPC) and lipoaspirate derived mesenchymal stromal cells (adipose stromal cells, ASC) cultivated either in normal (1g/l) or high (4.5g/l) glucose medium to identify similarities and discrepancies and elucidate high glucose effects considering cell-based therapies in diabetic retinopathy. A hallmark of diabetic retinopathy is pericyte- dropout increased vascular permeability and progressive vascular occlusion. ASC and HRMVPC were cultured in NG or HG for 7 days. Then cells were trypsinized, washed and RNA isolated using Qiagen RNeasy mini Kit (Qiagen, Hilden, Germany). RNA quality was tested by capillary electrophoresis on an Agilent 2100 Bioanalyzer (Agilent) and high quality (RNA integrity values ≥ 9) was confirmed.

2020-05-26 | GSE144605 | GEO

Expression data from ex vivo and cultured adipose-derived mesenchymal stromal cells (AdMSC)

Project description:Expression data from ex vivo and cultured adipose-derived mesenchymal stromal cells (AdMSC)

| PRJNA562899 | ENA

Functional comparison between healthy and multiple myeloma adipose stromal cells

Project description:Multiple myeloma (MM) is a not curable B-cell neoplasia characterised by the accumulation of tumor plasma cells within the bone marrow. As a consequence, 80% of patients develop bone osteolytic lesions that remain even after complete remission. We and others had demonstrated that bone marrow derived Mesenchymal Stromal Cells (MSC) are abnormal in MM and thus could not be used for an autologous treatment to repare bone damages. Adipose Stromal Cells (ASC) represent an interesting alternative to MSC for cellular therapy. Thus, in this paper we asked if they could be a good candidate in repairing MM bone lesions. For the first time, we present a transcriptomic, phenotypic and functional comparison between ASC from MM patients and healthy donors (HD) relying on their autologous MSC counterpart. In contrast to MM MSC, MM ASC do not exhibit major abnormalities. However, the changes observed in MM ASC and the supportive property of ASC on MM cells question their putative and safety uses at autologous or allogenic level.

2019-06-27 | GSE133346 | GEO

Expression data from ex vivo and cultured adipose-derived mesenchymal stromal cells (AdMSC)

Project description:The effect of standard tissue culture on AdMSC is not well defined, therefore we sought to analyse global gene changes when a defined ex vivo population of AdMSC was subjected to standard tissue culture conditions.

2019-08-30 | GSE136633 | GEO

Gene expression profiles of human retinal microvascular pericytes (HRMVPC) and human lipoaspirate derived mesenchymal stromal cells (adipose stromal cells, ASC)

Project description:Gene expression profiles of human retinal microvascular pericytes (HRMVPC) and human lipoaspirate derived mesenchymal stromal cells (adipose stromal cells, ASC)

| PRJNA604188 | ENA

Exome sequencing of human ex vivo ovarian cancer models with matched stromal cells

Project description:Ascites or solid tumour from patients with ovarian cancer was collected and grown in culture as ex vivo models. Each sample has a mixture of tumour and stromal cells which were separated into individual cultures. Therefore each patient has tumour and stromal cultures originating from the same tissue collection. Variant calling (exome-seq) analysis was performed on these matched models to establish tumour specific mutations. Stromal cells were used to rule out germline mutations.

2020-03-13 | E-MTAB-7225 | biostudies-arrayexpress

ASC deficiency suppresses proliferation and prevents medulloblastoma incidence

Project description:Apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) is silenced by promoter methylation in many types of tumors, yet ASC’s role in most cancers remains unknown. Here, we show that ASC is highly expressed in a model of medulloblastoma, the most common malignant pediatric brain cancer. Importantly, while ASC deficiency did not affect normal cerebellar development, ASC knock-out mice in the Smoothened (ND2:SmoA1) transgenic model of medulloblastoma exhibited a profound reduction in medulloblastoma incidence and delayed tumor onset. Premalignant lesions in cerebella of ASC-/-;ND2:SmoA1 mice displayed a striking decrease in number of ectopic progenitors. While proliferation rates decreased with ASC deletion, apoptosis and differentiation markers remained unchanged. Interestingly, ASC deficiency disrupted expression of genes in the TGF-ß pathway and increased the level of nuclear Smad3 in this medulloblastoma model. Together, these results demonstrate an unexpected requirement for ASC in Sonic hedgehog-driven medulloblastoma tumorigenesis, thus identifying ASC as a promising novel target for anti-tumor therapy. reference x sample

2014-05-23 | E-GEOD-48682 | biostudies-arrayexpress

Identification of ASC-interacting proteins

Project description:To explore the spatiotemporal regulation of ASC speck formation and inflammasome activation, we infected primary WT and Asc–/– bone marrow-derived macrophages (BMDMs) with the bacterium Francisella novicida to induce AIM2 inflammasome activation and then performed ASC IP-MS to identify proteins that interacted with ASC. We compared the IP products between WT BMDMs and Asc–/– BMDMs, and found that many proteins specifically interacted with ASC.

2024-05-29 | PXD052497 | Pride

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