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Putative Allergens Identified in Mango (Mangifera indica Linn) leaf and fruit with transcriptome analysis

ABSTRACT: Putative Allergens Identified in Mango (Mangifera indica Linn) leaf and fruit with transcriptome analysis

PROVIDER: PRJNA597009 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10746580_1.fastq.gz	Fastqsanger.gz
	SRR10746580_2.fastq.gz	Fastqsanger.gz
	SRR10746581_1.fastq.gz	Fastqsanger.gz
	SRR10746581_2.fastq.gz	Fastqsanger.gz

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Putative Allergens Identified in Mango (Mangifera indica Linn) leaf and fruit with transcriptome analysis

Project description:Purpose: this study provided a comprehensive sequence for a systemic view of the transcriptome between mango leaf and fruit, as well as fruit allergens, which will be useful for further genomic research studies and breeding of lower allergenic mango cultivars. Methods:Some allergens have previously been identified in mango (Mangifera indica Linn), including profilins, Bet v 1-like proteins and chitinase. In this paper, 66 potential allergen genes were identified and their relative expressions evaluated in mango fruit and leaf using Illumina RNA-Seq technology. Results:A total of 17.63Gb Clean Data was obtained.The number of %≥Q30 was above 94.58%.RNA-Seq generated 11,751,123 contigs that were assembled into 99,328 unigenes with 16,848 unigenes of >1000 bp. A total of 230,242 unigenes were annotated using public protein databases, with a cut-off E-value above 10−5, of which 27,295, 46,030, 24,227 and 14,023 unigenes were assigned to gene ontology terms, Nr, Swiss-Prot and clusters of orthologous groups, respectively. Allergens mainly belonged to pollen allergen, pathogenesis-related protein Bet v I family and NADPH-dependent FMN reductase.

2020-12-01 | GSE142427 | GEO

Transcriptome and Proteomic Analysis of Mango (Mangifera indica Linn) Fruits

Project description:Transcriptome and Proteomic Analysis of Mango (Mangifera indica Linn) Fruits

2015-02-20 | PXD000808 | Pride

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Transcriptome Analysis of Mango (Mangifera indica L.) Fruit Epidermal Peel to Identify Putative Cuticle-Associated Genes.

Project description:Mango fruit (Mangifera indica L.) are highly perishable and have a limited shelf life, due to postharvest desiccation and senescence, which limits their global distribution. Recent studies of tomato fruit suggest that these traits are influenced by the expression of genes that are associated with cuticle metabolism. However, studies of these phenomena in mango fruit are limited by the lack of genome-scale data. In order to gain insight into the mango cuticle biogenesis and identify putative cuticle-associated genes, we analyzed the transcriptomes of peels from ripe and overripe mango fruit using RNA-Seq. Approximately 400 million reads were generated and de novo assembled into 107,744 unigenes, with a mean length of 1,717 bp and with this information an online Mango RNA-Seq Database (http://bioinfo.bti.cornell.edu/cgi-bin/mango/index.cgi) which is a valuable genomic resource for molecular research into the biology of mango fruit was created. RNA-Seq analysis suggested that the pathway leading to biosynthesis of the cuticle component, cutin, is up-regulated during overripening. This data was supported by analysis of the expression of several putative cuticle-associated genes and by gravimetric and microscopic studies of cuticle deposition, revealing a complex continuous pattern of cuticle deposition during fruit development and involving substantial accumulation during ripening/overripening.

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