Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing Facilitates Quantitative Analysis of Spermidine Inhibited Transcriptomes

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of Spermidine Inhibited Transcriptomes

PROVIDER: PRJNA597047 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10748849_1.fastq.gz	Fastqsanger.gz
	SRR10748849_2.fastq.gz	Fastqsanger.gz
	SRR10748850_1.fastq.gz	Fastqsanger.gz
	SRR10748850_2.fastq.gz	Fastqsanger.gz
	SRR10748851_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Next Generation Sequencing Facilitates Quantitative Analysis of Spermidine Inhibited Transcriptomes

Project description:Purpose: Spermidine inhibits the activation of interferon primed dendritic cells. The goals of this study are to identify genes and pathways invovled in the regulation. Methods: Mouse monocyte derived dendritic cells were primed with interferon α for 24 hours, and then cultured with R837, together with or without spermidine for 18 hours. Then, the next-generation libraries of mRNA were prepared using VAHTS mRNA-seq v2 Library Prep Kit for Illumina® (Vazyme, Nanjing, China). The Library quality was determined by Bioanalyzer 4200 (Agilent, Santa Clara, CA, USA). Then the mRNA-seq libraries were sequenced in HiSeq X10 system (Illumina, San Diego, CA, USA) on a 150bp paired-end run. The differentially expressed genes were selected as having more than 1 fold difference in their geometrical mean expression between the compared groups and a statistically significant p-value (<0.05) by analysis of DEseq2. The GO analysis on differentially expressed genes was performed with an R package: Clusterprofiler using a p<0.05 to define statistically enriched GO categories. Pathway analysis was used to determine the significant pathway of the differential genes according to Kyoto Encyclopedia of Genes and Genomes Database (http://www.genome.jp/kegg/) and DAVID Bioinformatics Resources 6.8 (https://david.ncifcrf.gov/). Results: Genes in the JAK/STAT pathway and the NF-κB pathway were inhibited by spermidine. As a result, the pro-inflammatory cytokines including IL-1β, IL-12a, IL-12b (IL-12/23 p40), IL-6 and IL-23a decreased obviously upon spermidine treatment

2019-12-21 | GSE142452 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of MicroRNA-148a Inhibited Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of MicroRNA-148a Inhibited Transcriptomes

| PRJNA597230 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Solanum lycopersicum bud and flower Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Solanum lycopersicum bud and flower Transcriptomes

2021-02-27 | GSE164382 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of IBP Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of IBP Transcriptomes

| PRJNA761350 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Retinoblastoma Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Retinoblastoma Transcriptomes

| PRJNA436090 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of CHAA Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of CHAA Transcriptomes

| PRJNA725272 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Osteoblast Mineralization Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Osteoblast Mineralization Transcriptomes

| PRJNA623508 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Ter cells Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Ter cells Transcriptomes

| PRJNA430875 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Chenopodium quinoa Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Chenopodium quinoa Transcriptomes

| PRJNA658178 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Tartary buckwheat Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Tartary buckwheat Transcriptomes

| PRJNA522429 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data