Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Integrated Multi-Omics Analysis Reveals Glucose Metabolic Reprogramming and Identifies a Novel Hexokinase in Alcoholic Hepatitis

ABSTRACT: Integrated Multi-Omics Analysis Reveals Glucose Metabolic Reprogramming and Identifies a Novel Hexokinase in Alcoholic Hepatitis

PROVIDER: PRJNA597328 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10759848_1.fastq.gz	Fastqsanger.gz
	SRR10759848_2.fastq.gz	Fastqsanger.gz
	SRR10759849_1.fastq.gz	Fastqsanger.gz
	SRR10759849_2.fastq.gz	Fastqsanger.gz
	SRR10759850_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 56

Similar Datasets

Integrated Multi-Omics Analysis Reveals Glucose Metabolic Reprogramming and Identifies a Novel Hexokinase in Alcoholic Hepatitis

Project description:Purpose: We recently demonstrated that alcoholic hepatitis (AH) is characterized by de-differentiation of hepatocytes and loss of mature functions. Glucose metabolism is tighly regulated in healthy hepatocytes. We hypothesize that AH may lead to metabolic reprogramming of the liver, including dysregulation of glucose metabolism. Methods: We performed integrated metabolomic and transcriptomic analyses of liver tissue from patients with AH (n=13), alcoholic cirrhosis (n=10) or normal liver tissue from hepatic resection (n=16). Focused analyses of chromatin immunoprecipitation coupled to DNA sequencing (ChIP-seq) was performed. Functional in vitro studies were performed in primary rat and human hepatocytes and HepG2 cells. Results: Patients with AH exhibited specific changes in the levels of intermediates of glycolysis/gluconeogenesis, the TCA cycle, and monosaccharide and disaccharide metabolism. Integrative analysis of the transcriptome and metabolome revealed the utilization of alternate energetic pathways, metabolite sinks and bottlenecks, and improper glucose storage in AH patients. Among genes involved in glucose metabolism, hexokinase domain containing 1 (HKDC1) was identified as the most up-regulated kinase in AH patients. Histone active promoter and enhancer markers were increased in HKDC1 genomic region. High HKDC1 levels were associated with the development of acute kidney injury and decreased survival. Increased HKDC1 activity contributed to the accumulation of G6P and glycogen in primary rat hepatocytes. Conclusion: Altered metabolite levels and mRNA expression of metabolic enzymes suggest the existence of profound reprogramming of glucose metabolism in AH. Increased HKDC1 expression may contribute to dysregulated glucose metabolism and may serve as a novel biomarker and therapeutic target for AH.

2021-07-09 | GSE142530 | GEO

Pathogenesis of alcoholic hepatitis

Project description:Pathogenesis of alcoholic hepatitis

| PRJNA704267 | ENA

Hepatic cell proliferation and outcome in alcoholic hepatitis: histology, gene expression and effect of stem cell therapy

Project description:Regeneration and alcoholic hepatitis

2014-08-31 | E-MTAB-2664 | biostudies-arrayexpress

Plasma Protein Signatures in Alcoholic Hepatitis

Project description:Supporting plasma proteomic data from clinical patients with alcoholic hepatitis in comparison to relevant controls and across treatment time points, baseline, 28/29 days, 12 weeks. Samples were digested with trypsin, labeled with TMT 10-Plex, then analyzed by LC-MS/MS. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline.

2019-11-01 | MSV000084528 | MassIVE

Silent Alcoholic Steatohepatitis vs Alcoholic Hepatitis: Clinical, Histological and Transcriptome Analysis

Project description:Silent Alcoholic Steatohepatitis vs Alcoholic Hepatitis: Clinical, Histological and Transcriptome Analysis

| PRJNA635585 | ENA

INTEAM CONSORTIUM - ALCOHOLIC HEPATITIS LIVER RNA SEQUENCING

Project description:INTEAM CONSORTIUM - ALCOHOLIC HEPATITIS LIVER RNA SEQUENCING

| PRJNA531222 | ENA

INTEAM CONSORTIUM - ALCOHOLIC HEPATITIS LIVER RNA SEQUENCING

Project description:INTEAM CONSORTIUM - ALCOHOLIC HEPATITIS LIVER RNA SEQUENCING

| PRJNA531223 | ENA

Super Enhancer Regulation of Cytokine-Induced Chemokine Production in Alcoholic Hepatitis [RNA-Seq]

Project description:Super enhancer in liver propagates inflammatory signaling by super enhancer-mediatedinducing chemokine expression and is the therapeutic potential of BET inhibition in Alcoholic hepatitis(AH) treatment.

2020-10-19 | GSE155907 | GEO

Transcriptome profiles of liver biopsy tissues from sever alcoholic hepatitis patients (derivation cohort)

Project description:Corticosteroids are the current standard of care to improve short-term mortality in severe alcoholic hepatitis (AH), although nearly 40% of the patients do not respond and accurate pre-treatment predictors are lacking. We developed 123-gene prognostic score based on molecular and clinical variables before initiation of corticosteroids. Furthermore, The gene signature was implemented in an FDA-approved platform (NanoString), and verified for technical validity and prognostic capability. Here we demonstrated that a Nanostring-based gene expressoin risk classificatoin is useful to predict mortality in patients with severe alcoholic hepatitis who were treated by corticosteroid

2018-02-18 | GSE94397 | GEO

Transcriptome profiles of liver biopsy tissues from sever alcoholic hepatitis patients (validation cohort, Brussels)

Project description:Corticosteroids are the current standard of care to improve short_term mortality in severe alcoholic hepatitis (AH), although nearly 40% of the patients do not respond and accurate pre_treatment predictors are lacking. We developed 123_gene prognostic score based on molecular and clinical variables before initiation of corticosteroids. Furthermore, The gene signature was implemented in an FDA_approved platform (NanoString), and verified for technical validity and prognostic capability. Here we demonstrated that a Nanostring_based gene expressoin risk classificatoin is useful to predict mortality in patients with severe alcoholic hepatitis who were treated by corticosteroid

2018-02-18 | GSE94399 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data