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Transcriptome profiling (RNA-seq) analysis of control and NaIO3-treated RPE cells

ABSTRACT: Transcriptome profiling (RNA-seq) analysis of control and NaIO3-treated RPE cells

PROVIDER: PRJNA597602 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10766046_1.fastq.gz	Fastqsanger.gz
	SRR10766046_2.fastq.gz	Fastqsanger.gz
	SRR10766047_1.fastq.gz	Fastqsanger.gz
	SRR10766047_2.fastq.gz	Fastqsanger.gz
	SRR10766048_1.fastq.gz	Fastqsanger.gz

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Transcriptome profiling (RNA-seq) analysis of control and NaIO3-treated RPE cells

Project description:Purpose: The goal of this experiment is to investigate the relative contributions of various types of cell death in NaIO3-treated RPE cells Methods: RPE cell mRNA profiles were generated by deep sequencing, in triplicate, using Illumina HiSeq 4000. Results: According to the differential expressions of mRNA genes, among which 661 genes were upregulated and 637 genes were downregulated with significance after NaIO3 treatment . From the Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, it has been identified that ferroptosis ranked as the top pathway in comparison to other types of cell death (apoptosis, and necroptosis) upon NaIO3 treatment, which was additionally validated by the higher FPKM of main regulators in ferroptosis than that in apoptosis. Additionally, it was found that heme oxygenase-1 (HMOX1, also known as HO-1) as well as SLC7A11 (solute carrier family 7 member 11) mRNA were characterized as the most dramatically upregulated genes upon NaIO3 treatment against ARPE-19 cells Conclusions: Our study represented that ferroptosis serves as a predominant pathogenic factor involved in AMD pathological process.

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Transcriptome profiling (RNA-seq) analysis of NaIO3-induced RPE cells with or witout CaPB treatment

Project description:Purpose: The goal of this experiment is to investigate the role of CaPB in NaIO3-induced RPE cells Methods: RPE cell mRNA profiles were generated by deep sequencing, in triplicate, using Illumina HiSeq 4000. Results: According to the differential expressions of mRNA genes, among which 463 genes were upregulated and 397 genes were downregulated with significance in NaIO3-induced RPE cells after CaPB treatment . From the Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, it has been identified that ferroptosis ranked as the most downregulated pathway upon CaPB treatment. Conclusions: Our study represented that CaPB against NaIO3-treated RPE cell death via ferroptosis inhibition

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Project description:m6A-mRNA&lncRNA Epitranscriptomic Microarray of primary mouse RPE cells comparing control untreated RPE cells with RPE cells treated with TGF-β2 at a concentration of 10 ng/ml. The goal was to determine the effects of RNA m6a methylation on primary mouse RPE cells undergoing epithelial-mesenchymal transition induced by TGF-β2.

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RFX7, ANKRA2, and p53-regulated transcriptome in RPE-1

Project description:Identification of the RFX7-regulated transcriptome in Nutlin-3a and DMSO control treated RPE-1 cells

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Project description:QuantSeq from MCF-7, U2OS, and RPE-1 cells treated with Nutlin-3a or DMSO control

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