Project description:The objective of this study was to compare liver gene expressions in bovine fetuses derived from somatic cell nuclear transplantion (NT) with those in the liver of bovine fetuses derived from artificial insemination (AI) and in vitro fertilized (IVF) eggs. Gene expression profiles in the liver of an NT-calf was also compared with those in the liver of AI-calf. Keywords = nuclear transplantation Keywords = bovine Keywords = liver gene expression Keywords = fetus Keywords: parallel sample
Project description:Harnessing maternal blood to predict fetal growth is an emerging area in livestock research, offering a non-invasive approach to monitor fetal development and potentially enhance reproductive management in cattle. In this study, blood samples were collected from 20 recipient cows carrying female fetuses produced via in vitro embryo transfer at 63 days of gestation. At birth, calf weights ranged from 30 to 72.5 kg, with an average of 45 kg. RNA-seq analysis of maternal blood revealed transcriptomic patterns associated with calf birth weight. Furthermore, a transcriptomic signature was identified that enables accurate prediction of calf birth weight with low error.
Project description:Histones were isolated from brown adipose tissue and liver from mice housed at 28, 22, or 8 C. Quantitative top- or middle-down approaches were used to quantitate histone H4 and H3.2 proteoforms. See published article for complimentary RNA-seq and RRBS datasets.
Project description:The first GSSM of V. vinifera was reconstructed (MODEL2408120001). Tissue-specific models for stem, leaf, and berry of the Cabernet Sauvignon cultivar were generated from the original model, through the integration of RNA-Seq data. These models have been merged into diel multi-tissue models to study the interactions between tissues at light and dark phases.