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Identification of an ECF sigma factor that facilitates electroactive biofilm formation by Shewanella oneidensis MR-1

ABSTRACT: Identification of an ECF sigma factor that facilitates electroactive biofilm formation by Shewanella oneidensis MR-1

PROVIDER: PRJNA597846 | ENA |

REPOSITORIES: ENA

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Identification of an ECF sigma factor that facilitates electroactive biofilm formation by Shewanella oneidensis MR-1: Experiment 2

Project description:Shewanella oneidensis MR-1 was grown on electrodes in electrochemical flow cells (EFC), and the transcriptome profiles of electrode-attached MR-1 cells were compared under electrolyte-flow and static (non-flow) conditions. The results revealed that the SO_3096 gene encoding a putative extracytoplasmic function (ECF) sigma factor, as well as genes related to cyclic-di-guanosine monophosphate and flagella synthesis (e.g., SO_3556 and flrC) and c-type cytochrome maturation (dsbD), was significantly up-regulated under the electrolyte-flow condition. Compared to wild-type MR-1 (WT), a deletion mutant of SO_3096 (∆SO_3096) showed an impaired biofilm formation and a decreased current generation in EFC, suggesting that the ECF sigma factor encoded by this gene is involved in the regulation of biofilm formation and current generation under electrolyte-flow conditions. We also compared the transcriptome profiles of WT and ∆SO_3096 grown in EFC, confirming that many genes up-regulated under the electrolyte-flow condition, including dsbD, were down-regulated in ∆SO_3096. Transcription analysis using lacZ as a reporter gene showed that the promoter of dsbD is activated in the presence of SO_3096. Measurement of current generation by a dsbD-deletion mutant revealed that this gene is essential for electron transfer to electrodes. These results suggest that the SO_3096 protein serves as an ECF sigma factor that regulates cellular functions related to electroactive biofilm formation under electrolyte-flow conditions.

2020-06-01 | GSE142642 | GEO

Identification of an ECF sigma factor that facilitates electroactive biofilm formation by Shewanella oneidensis MR-1: Experiment 1

2020-06-01 | GSE142641 | GEO

Identification of an ECF sigma factor that facilitates electroactive biofilm formation by Shewanella oneidensis MR-1: Experiment 2

Project description:Identification of an ECF sigma factor that facilitates electroactive biofilm formation by Shewanella oneidensis MR-1: Experiment 2

| PRJNA597849 | ENA

Identification of an ECF sigma factor that facilitates electroactive biofilm formation by Shewanella oneidensis MR-1: Experiment 1

Project description:Identification of an ECF sigma factor that facilitates electroactive biofilm formation by Shewanella oneidensis MR-1: Experiment 1

| PRJNA597850 | ENA

Transcript structures in Shewanella oneidensis MR-1

Project description:5' RNA-Seq of mRNA from S. oneidensis MR-1 grown aerobically in defined lactate medium

2012-07-19 | GSE39474 | GEO

Quantitative proteome of Shewanella oneidensis MR-1

Project description:We investigated the anode-specific responses of Shewanella oneidensis MR-1, an exoelectroactive ammaproteobacterium, using for the first time iTRAQ and 2D-LC MS/MS driven membrane proteomics to compare protein abundances in S. oneidensis when generating power in MFCs, and growing in a continuous culture.

2016-12-08 | PXD004090 | Pride

Transcript structures in Shewanella oneidensis MR-1

Project description:5' RNASeq of mRNA from S. oneidensis MR-1 grown aerobically in Luria-Bertani broth (LB) and defined lactate minimal medium

2013-03-21 | GSE45313 | GEO

Regulation of Energy Metabolism by the Extracytoplasmic Function (ECF) σ factors of Arcobacter butzleri

Project description:The extracytoplasmic function (ECF) σ factors are fundamental for bacterial adaptation to distinct environments and for survival under different stress conditions. The emerging pathogen Arcobacter butzleri possesses seven putative pairs of σ/anti-σ factors belonging to the ECF family. Here, we report the identification of the genes regulated by five out of the seven A. butzleri ECF σ factors. Three of the ECF σ factors play an apparent role in transport, energy generation and the maintainance of redox balance. Several genes like the nap, sox and tct genes are regulated by more than one ECF σ factor indicating that the A. butzleri ECF σ factors form a network of overlapping regulons. In contrast to other eubacteria, these A. butzleri ECF regulons appear to primarily regulate responses to changing environments in order to meet metabolic needs instead of an obvious role in stress adaptation.

2011-12-31 | GSE34089 | GEO

Comparison of gene expression and mutant fitness in Shewanella oneidensis MR-1

Project description:Comparison of gene expression and mutant fitness in Shewanella oneidensis MR-1

2012-07-19 | GSE39462 | GEO

A novel extracytoplasmic function (ECF) sigma factor regulates virulence in Pseudomonas aeruginosa

Project description:Next to the two-component and quorum sensing systems, cell-surface signaling (CSS) has been recently identified as an important regulatory system in Pseudomonas aeruginosa. CSS senses signals from outside the cell and transmits them into the cytoplasm. It consists of a TonB-dependent outer membrane receptor, a cytoplasmic membrane-localized sigma factor regulator (or anti-sigma factor), and an extracytoplasmic function (ECF) sigma factor. Upon perception of the extracellular signal by the receptor the ECF sigma factor is activated and promotes the transcription of a specific set of gene(s). Although most P. aeruginosa ECF sigma factors are involved in the regulation of iron uptake, we have identified a novel ECF sigma factor (PA0675) involved in the regulation of virulence. By microarray analysis of cells overexpressing PA0675 from the pMUM3 plasmid we have identified the genes regulated by this sigma factor.

2009-04-21 | GSE15697 | GEO

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